Study On Influence Of The Stimulation Of Micro DC On Biological Properties Of Osteoblasts In Vitro

Objective:By the stimulation of different intensitiesof direct current for osteoblasts cultured in vitro, the effect of micro direct current on proliferation and function of osteoblasts by has been studied. It provides the theoretical basis of cytology for application of direct current to promote alveolar bone growth for prosthodontics. Methods: In this paper we designe and develope the direct current stimulation device forosteoblasts in vitro: fixe the power, sliding rheostat, switch, and tin plates on a plexiglas plate 300mm×200mm, then connect the circuit together, punch two small holes on the lid which are corresponding to theplate hole. One end of the nickel-titanium arch wires is connected to the circuit , the other end is connected to the culture fluid power on and then the strength and stability are measured with a multimeter .We original cultured Sprague Dawley separated-training new bone rats' cranial parietal osteoblasts nestin with tissue mass methodtraining to the third generation, identified these cells by alkaline phosphatase dyeing. After the purification of osteoblast nestin training, the cells areexpand to the fourth generation or the fifth generation. We use these cells in the next step .In the first part of the experiment ,With 1x105 cells /hole in three groups of six vaccinations in training board, inoculated four holes per board. We divided the osteoblasts into three groups. The strength of Micro-current stimulation on the three groups of cells were 0μA,20μA and 100μA,stimulate three times everyday,to check the quantity of osteolasts of three groups on 1,3,5,7,9 day with MTT method,then repeat the experient two times,do statistic analysis with soft ware Spss16.0,draw the growth curves.In the second part of the experient ,cell group and the method of inoculation with the first step, test the activity of alkaline phosphatase of three groups on 1,3,5,7 day and repeat the experient two times, do statistic analysis with soft ware Spss16.0,draw histograms of ALP activity of osteoblasts. Results: This experiment osteoblast direct current developed by absolute error loading devices in 3μA;After the intensity of 20μA and 100μA direct current loading the proliferation of cells and control function was not statistically significant, three groups of cells proliferate fast in the first 3 ~ 5 days, slow down the next 2 days;The results between the osteoblasts and the control group in the the ALP activity of osteoblasts are statistically significantly different.There are some statistical differences between the intensity 20μA and 100μA. The ALP activity is better in the intensity 20μA group.Conclusion: The direct current of 20μA and 100μA has no effect for promoting cell proliferation. However, this direct current can increase the activity of ALP for osteoblasts. The activity of ALP for osteoblasts stimulated by direct current of 20μA was significantly better than the100μA direct current. The results proved that micro direct current can promote the activity of osteoblasts. By using the direct current of 20μA, the effect for promoting the activity of ALP for osteoblasts has been observed remarkably. It provided theoretical basis of cytology for application of direct current to promote alveolar bone growth for prosthodontics.