Effects Of Naringin On The Activity Of Alkaline Phosphatase And OPG?RANKL In Osteoblasts Stimulated By Lipopolysaccharide

ObjectivePeriodontitis is a chronic inflamatory disease,characterized by destruction of periodontal support tissue and absorption of alveolar bone.It is also the leading cause of tooth loss.In China,the incidence of periodontitis is high,and more common in adults,so periodontitis has become the most important factor affecting people's oral problems.The treatment of periodontitis is not only to remove the plaque and maintain good oral hygiene,but also to rebuild the damaged tissue and restore the occlusal function of teeth.Naringin is the main effective component of bubonic acid,which is effective in the treatment of osteoporosis and has some anti-inflammatory effects.Naringin has a proliferation effect on osteoblasts and can up-regulate the expression of osteoblast related factors.Under the condition of periodontal bone defect,naringin can promote the formation of new bone.But on the state of naringin in inflammation cases osteogenesis and related mechanism research also is less,so this study in vitro under lipopolysaccharide stimulation of MC3T3-E1 cells as the experimental model,using different concentrations of naringin as the research object,observe the different concentrations of naringin MC3T3-E1 cell proliferation and the impact of osteogenesis and naringin in inflammatory conditions inhibit bone absorption mechanism,in order to further apply naringin to bone resorption to provide theoretical basis for treatment of periodontitis.MethodsAfter subculture of primary MC3T3-E1 cells,the cells of the third generation were randomly divided into the control group and the experimental group.In the experimental group,the cells were divided into four groups according to the concentration of the drug and naringin.The LPS group had a final concentration of 1?g/m L LPS.In the low concentration group,the final concentration was 2 mg/L naringin +1 ?g/m L LPS.In the medium concentration group,the final concentration was 10 mg/L naringin +1 ?g/m L LPS.Group with high concentration of naringin: 20mg/L naringin +1 ?g/m L LPS;Blank control group: the same amount of DMSO was added,and no other treatment was done.Proliferation of osteoblasts was detected by cck-8 24 h later,alkaline phosphatase activity was detected 3 days later,and m RNA expression levels of osteoblasts osteocutrogenin(OPG)and nuclear factor k B receptor activator ligand(RANKL)were detected by RT-PCR.SPSS21.0 statistical software was used to analyze the data.ResultsCompared with LPS group,naringin group promoted the proliferation and differentiation of osteoblasts and the activity of ALP,and the difference was statistically significant(P<0.05).Compared with LPS group,all naringin group could up-regulate OPG m RNA level and reduce RANKL m RNA level,the difference was statistically significant(P<0.05).Among the three groups of naringin,2mg/L naringin solution promoted the osteogenic activity and inhibited the bone resorption ability most significantly(P<0.05).ConclusionsNaringin can promote the proliferation and differentiation of MC3T3-E1 cells stimulated by LPS,improve the activity of ALP,up-regulate the level of OPG m RNA,and reduce the level of RANKL m RNA.Therefore,Naringin may up-regulate the expression level of OPG and down-regulate the expression level of RANKL,inhibiting bone resorption and improving the osteogenic activity of osteoblasts.