Research Of Osteoporosis Rats Yishenjianpiliugao Serum Containing Effect On Osteoblasts

Objective: To investigate effection of the Yishen Jianpi liugao on rat osteoblast cell proliferation and differentiation. Method:SD rats injections of dexamethasone cause of senile osteoporosis model, given Yishen Jianpi liugao paste gavage, and set up the control group and the control group of western medicine, by gavage six weeks after fetching abdominal aorta blood, serum separation . Freshmen in vitro fetal rat osteoblast , and then use MTT (MTT) Determination of serum on osteoblasts in vitro proliferation role for the amino-phenol test than Lin Determination osteoblasts alkaline phosphatase ( ALP) activity, Alizarin red staining (ARS) study on osteoblast mineralization impact. At the same time detection NOS activity in cultured cells and NO concentration in the supernatant. Spleen and Kidney flow of cream in the treatment of senile osteoporosis mechanism for the serious impact on the health of the elderly common diseases provide new ideas and methods of treatment, so that they can further development and application of value. Results: yishenJianpiliugao containing serum take osteoblast proliferation obviously, and the control group compared with blank significant differences (P <0.05)Yishen Jianpi liugao containing serum have enhanced alkaline phosphatase activity and the promotion of the role of mineralized nodule formation (P <0.05).Conclusion:Yishen Jianpi liugao make osteoblast proliferation and differentiation significantly facilitating role.