HBV Infection With Different Immune Status And The Relationship Between The Expression Of SICAM-1

Objective Current HBV infection and the virus signs, indicators of immunization have had many reports, but with different immune status of the HBV infection and serum sICAM-1 expression in home and abroad has not been reported. Study at home and abroad to focus in the pathogenesis of HBV infection and the corresponding treatment, although made some progress, but the incidence in some specific areas is still not fully clear, this also brings some difficult to the consecutive treatment, therefore, the integration of the current domestic, international trends and our research base, according to Western classification of disease diagnosis, through different HBV infection immune status of the investigation in Guangxi region, also detect the expression of sICAM-1 to explore the different immune status and HBV infection serum sICAM-1 expression, can provide a theoretical basis for clinical treatment.Method Enzyme-linked immunosorbent assay (ELISA) determination of sICAM-1 levels.①Package: 0.05M PH9.9 carbonate coat with a buffer, the antibody is diluted to the protein content of 1 ~ 10μg/ml. In response to each polystyrene plate holes plus 0.1ml, 4℃overnight. The next day, discard hole solution, wash with washing buffer 3 times, each time 3 minutes. (The washing, the same below).②Sample: Add some diluted 0.1ml sample to be tested at the above reaction has been coated holes, set at 37℃for 1 hour. Then wash. (And do a blank hole, a positive control hole and a negative control hole).③Plus labeled antibody: in the reaction of the hole, adding fresh dilution of the labeled antibody (after dilution by titration) 0.1ml, 37℃0.5 ~ 1 hour incubation and washing.④Color LCD monitor plus substrate: the reaction of the hole in the provisional prepare TMB substrate solution 0.1ml, 37℃10 ~ 30 minutes.⑤Termination reaction: the reaction in the hole by adding 2M sulfuric acid 0.05ml.⑥Evaluation of results: to be a white background, direct observation with the naked eye: the deeper the color reaction hole, the stronger the degree of positive and negative reaction is a colorless or very light, according to the depth of color was to "+"," - "sign indicates. OD values can be measured: in the ELISA test apparatus, at 450nm (if ABTS color, then 410nm) department, to zero after the control test hole OD value of each hole, if greater than the negative control OD values under 2.1 times, as the positive.Results①With the measurement data of two sample t test statistics indicates that immune tolerance and sICAM-1 levels in healthy population have no significant difference (P> 0.05).②With the measurement data of two sample t test statistics indicates that immune clearance and sICAM-1 levels in healthy population are different (P <0.05).③With the measurement data of two sample t test statistics indicates that immune deficiency and sICAM-1 levels in healthy population have no significant difference (P> 0.05).④Variety of measurement data with the average number of F test statistics indicates that immune clearance, immune tolerance and immune deficiency sICAM-1 levels have differences (P <0.05), while immune tolerance and immune deficiency have no significant differences (P> 0.05).Conclusions①Immune clearance sICAM-1 expression is higher than those of healthy control group.②Immune tolerance and immune deficiency sICAM -1 expression are no significant differences than those of healthy control group.③Immune clearance sICAM-1 expression is higher than those of immune tolerance and immune deficiency,while those of immune tolerance and immune deficiency have no significant differences.