The Chinese Herbal Compound Effect On Spleen-deficiency AR Rats Nasal Mucosa IL-2, β1-Defensin MRNA And Serum IgE Expression

Objective:Allergic rhinitis is the medical "Bi Qiu." My teacher dajing.xiong is good at curing it from the spleen, the clinical efficacy rather, it is through animal experiments, from the pathological, biochemical and molecular biology, and explore Chinese herbal compound on rats with spleen deficiency type AR-relatedindicators.Methods:HealthySDratswererandomlydividedinto7groups.①normalgro up②spleen-deficiencyARgroup③spleen-deficiencyARMedicinesgroup④spleen-defic iency AR WM group⑤AR group⑥AR Medicines⑦AR WM group. Section and fatigue with diet methods passes animal model to establish spleen deficiency, according to An Yunfang Methods reported allergic rhinitis. Drug intervention:In addition to the blank group, spleen-deficiency AR group, AR group, the rest of the group in the local excitation at the same time each day to be the corresponding drug intervention, respectively, administered 1 hour before to be 5% of the egg albumin solution.From the first day of modeling, observing animal behavior, status, signs, feeding, drinking water, toilet characters, and so on, within 30 minutes after excitation focused on nasal symptoms observed, namely, nasal itching, sneezing, nasal discharge, etc, and After the shot at the end Right nasal symptoms score. Drug seven days later, rats were detected by HE staining septum mucosa, serum IgE, IL-2 mRNA,β1-Defensin mRNA and other changes.Results:Spleen AR group and the AR group were 5 points above integral spleen AR group compared with the AR group points are different. Medication after seven days of nasal treatment groups compared with the model group score difference (p <0.05), compared with the control group no significant difference (p> 0.05). The traditional Chinese medicine and western medicine group and the treatment group compared with the corresponding nasal score was no difference (p> 0.05).Serum IgE: Spleen AR group and the AR groups compared with the control group there was significant difference (p<0.01).Spleen AR group compared with the AR group significant difference (p<0.05); the treatment group compared with the AR group significant difference (p<0.01); compared with the control group no significant difference (P>0.05) IL-2 mRNA expression:Spleen AR group and the AR groups compared with the control group there was significant difference (p<0.01). Spleen AR group compared with the AR group significant difference (p<0.05); the treatment group compared with the AR group significant difference (p<0.01), compared with the control group no significant difference (P>0.05).β1-Defensin mRNA expression: Spleen AR group and the AR groups compared with the control group there was significant difference (p<0.01).Spleen AR group compared with the AR group significant difference (p<0.05); the treatment group compared with the AR group significant difference (p<0.01), compared with the control group no significant difference (P>0.05).Conclusion:The spleen deficiency can aggravate nasal symptoms of AR rats. Chinese medicine can significantly improve the nasal mucosa pathological response; and through multi-level, multi-angle on the regulation of cytokine mRNA expression, can play a very good preventive effect.