| Objective:To study the Tongmai Dasheng pill action mechanism on renal deficiency induced anovulation infertility disorder rats, and to clarify applying Chinese herbs to treat the renal deficiency induced anovulation infertility is validity.Methods:Renal deficiency induced anovulation infertility rats were randomly divide d into seven groups, the Tongmai Dasheng pill high-dose group, Tongmai Dasheng pill medium-dose group, Tongmai Dasheng pill low-dose group, chlorine clomifene group Yougui pill group, model group, control group. At the first 70 days, rat vagina open, consecutive vaginal smears of two cycles (4-5 days a cycle), if the vaginal epith elium was sustained keratoses without cyclical changes, suggesting that the model was successful, the other rats were removed.At the 80 days, after 5 weeks continuous fed, the animals were killed,and took blood quickly from femoral artery, stripped rat ovary, uterus. Supernatant after centrifugation of blood, placed in -20℃. Refrigerator, keep it for later testing. Rat vaginal exfoliated cells were detected by Papanicolaou st ain; After formaldehyde fixation, dehydration embedding, HE staining, look at the ut erine, ovarian morphology under the light microscopy.Apply SP immunohistochemi cal technique way to detecte rat ovary IGF-1R expression. Apply ELISA assay way to detect serum FSH/LH/IGF-1 level, RI way to detecte serum E2/T content, statistical analysis of all data are in complete support of SPSS 13.0 software.Results:Compared with Tongmai Dasheng pill group and model group, rats had a reg lar estrous cycle, uterus/ovary morphology showed that Tongmai Dasheng pill group uterus endometrial became thicker, the number of glands is more than the model grou p(P<0.01), endometrium transformation from proliferative to secretory phase; Com pared with model group, pathologic cystic ovarian follicles decreased, small amouts mature follicles and corpus luteum formation. Tongmai Dasheng pill can increase serum FSH, LH level (P<0.05), has a trend to elevate serum E2 level,but the difference was not significantly(P>0.05), and can decrease serum T level (P<0.05, Tongmai Dasheng pill aslo can reduce the serum level of IGF-1 and IGF-1R expression in the ovary (P<0.05)Conclusion:1. Intramuscular injected testosterone female rats ovulatory infertility reproductive function decreased, showing persistent vaginal exfoliated cells keratosis, endometrium presents proliferative phase, ovary presents pathologic cystic follicles, no mature follicle and corpus luteum generated. After modeling, the serum E2, FSH, LH levels become lower, T increased. Serum IGF-1 levels abnormal increased, ovarian local IGF-1R also expression in abnormal increase.2. Tongmai Dasheng pill can improve renal deficiency induced anovulation infertility disorder rats uterine and ovarian pathology and retrieve the body sex hormones levels. Tongmai Dasheng pill can increase the number of endometrial glands, increase endometrial thickness, and promote from the proliferative endometrium into a secretory phase; Tongmai Dasheng pill can reduce the pathologic cystic follicle number, small amouts mature follicles and corpus luteum formation, can promote follicular development,maturation and discharge; Tongmai Dasheng pill can increase serum FSH, LH level, has the trend of increase serum E2 level, can decrease serum T level, can improve the pituitary and ovarian endocrine function.3..Tongmai Dasheng pill had a impaction to regulate on renal deficiency induced anovulation infertility disorder rats ovary local IGF-1/IGF-1R factor. Tongmai Dasheng pill also can reduce serum high IGF-1 level, decrease ovarian local high IGF-1R expression.4. Traditional Chinese Herbs treatment action on renal deficiency induced anovulation infertility disorder rats, can achieve from regulating local ovarian factors andhormone.
|
PDF Full Text Request