The Study On The Influences Of Expression Level Of Infertility Rats Ovary Smad3、P-Smad3in Cases Of Ovulation Disorders Caused By Kidney Deficiency Through BuShenTiaoChong

Objective:In previous research, on the basis of further study on "BuShenTiaoChong" legislation Tongmai Dasheng pill of big rat ovarian TGF-β/Smads signal transduction pathways, and to explore Chinese medicine "BuShenTiaoChong" method on the mechanism of action of type kidney ovulation disorder infertility.Method:select SD rats,9age for intramuscular injection of testosterone propionate to establish kidney ovulation disorder infertility in rats (ASR) model, through observation of the rat vaginal exfoliated cells smear, selecting and building successful, rats were randomly divided into seven groups, the normal group, model group, the Tongmai Dasheng pill high-dose group, Tongmai Dasheng pill medium-dose group, Tongmai Dasheng pill low-dose group, letrozole group, Yougui pill group. On80days of age to rats, interfere in with drug five weeks successively, then take blood femoral artery and kill animals, quickly stripped rats ovaries to storage in liquid nitrogen frozen. By protein immunoblot method, examine each rat ovarian local Smad3and P-Smad3expression quantity.Results:1、compared with model group, normal group, Tongmai Dasheng pill high-dose group, Tongmai Dasheng pill medium-dose group, the group of letrozole group rats, the expression level of ovarian Smad3is higher, there are significant difference (P<0.01), Tongmai Dasheng pill low-dose group, Yougui pill group.smad3also was increased, the difference was statistically significant (P<0.05); Tongmai Dasheng pill All dose groups, levels of Smad3in Tongmai Dasheng pill high-dose group and Tongmai Dasheng pill medium-dose group were higher than that of Tongmai Dasheng pill low-dose group.2、compared with model group, normal group, the group of letrozole group, Yougui pill group, rats ovaries P-Smad3higher expression level, with significant difference (p<0.01), and lower in the group, group of Smad3levels also was increased, the difference was statistically significant (p<0.05); Tongmai Dasheng pill All dose groups, levels of P-Smad3in Tongmai Dasheng pill high-dose group was higher than that of Tongmai Dasheng pill low-dose group.and Tongmai Dasheng pill medium-dose group.Conclusion:1、Given intramuscular injection of testosterone propionate method successfully reproduce the type kidney ovulation disorder infertility in rats (ASR) model. The model can lead to a mature follicle and corpus luteum formation and pathologic cystic follicles.2、Large raw improve kidney ovulation disorder infertility unclogged arteries rats Smad3and P-Smad3expression level, thus improve ovarian local microenvironment.3、Unclogged arteries large born by local factors for ovarian Smad3and P-Smad3regulation to promote follicle growth, development and promotion of mature follicle rupture and eggs.4、Shows that Chinese medicine kidney DiaoChong France unclogged arteries big birth pills for kidney ovulation disorder infertility treatment effect, can be done by local factors regulate ovarian, especially on ovarian TGF-β/Smad signaling pathways control.