The Expression Of P53, VEGF And PCNA In Bladder Transitional Cell Carcinoma Tissue And Its Clinical Significance

ObjectiveTo study the expression of P53, vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) in bladder transitional cell carcinoma (BTCC) tissue and their relations with the pathological grades and clinical stages, and thus to determine the degree of bladder transitional cell carcinoma of differentiation, invasiveness and prognosis.Materials and MethodsTo collect transitional cell carcinoma of the bladder tissue paraffinum sample of 43 cases, from urinary surgery of chengde affiliated hospital medical college, which were validated with exairesis and pathematology, in March 2005～September 2006, of which 30 males and 13 females, aged 33～87 years old, with an average age of 65 years. Another to take 10 cases of normal bladder mucosa (taken from hyperplasia of prostate by open surgery) as the control group. To detection the expression of P53, VEGF and PCNA in the above-mentioned organization, using immunohistochemical SP method, combined with pathological and clinical data were analyzed statistically. The positive expression rates of P53, VEGF, PCNA in BTCC were 48.84%,67.44%, 62.79%. the negative expression of P53, VEGF, PCNA in normal cases with bladder issues, there was significant difference between normal bladder tissue and bladder transitional cell carcinoma tissues (p<0.05). The positive expression rates of P53 in bladder transitional cell carcinoma of the G1, G2, G3 grade were 12.67%, 54.17%, 85.71%, there was significant difference between G1 and G2, G3(P < 0.05), there was no significant difference between G2 and G3(P>0.05). The positive expression rates of P53 in the Tis～T1 and T2～T4 stage bladder transitional cell carcinoma were 38.24%, 88.89%, there was significant difference between Tis～T1 and T2～T4 stage(P<0.05).The positive expression rates of VEGF in the G1, G2, G3 grade bladder transitional cell carcinoma were 33.33%, 75.00%, 100%, there was significant difference between G1 and G2, G3(P < 0.05), there was no significant difference between G2 and G3(P>0.05). The positive expression rates of VEGF in the Tis~T1 and T2~T4 stage bladder transitional cell carcinoma were 58.82%, 100%, there was significant difference between Tis~T1 and T2~T4 stage(P<0.05). The positive expression rates of PCNA in the G1, G2, G3 grade bladder transitional cell carcinoma were 25.00%, 79.17%, 100%, there was significant difference between G1 and G2, G3(P<0.05), there was no significant difference between G2 and G3(P>0.05). The positive expression rates of PCNA in the Tis~T1 and T2~T4 stage bladder transitional cell carcinoma were 58.82%, 100%, there was significant difference between Tis~T1 and T2~T4 stage(P<0.05). The significantly correlation was noted between P53, VEGF and the pathological grades, clinical stages of BTCC(P<0.05). The significantly correlation was noted between PCNA and the pathological grades of BTCC(P<0.05), but PCNA was not related to the clinical stages(P>0.05).ConclusionsP53, VEGF, PCNA are closely related to the pathological grades and clinical stages of BTCC. P53, VEGF may serve as an important index to estimate the pathological grades, clinical stages and prognosis of BTCC. PCNA may serve as an auxiliary marker to estimate the pathological grades, clinical stages and prognosis of BTCC.