The Expression And Significance Of RECK, KISS-1 And MMP-2 In The In Endometriosis

Endometriosis is a common and popular gynecologic disease in women of the reproductive age. The main clinical symptoms are dysmenorrhea,chronic pain of pelvic cavity and infertility. It has the high invasion and recurrence. Its pattern of growth is similar to malignant disease. Recently the incidence of endometriosis has been distinctly increased. EMT seriously impacts the quality of life of reproductive women and their families,The exact mechanism of EMT is unknown.About the resource of the endometriosis have many theories such as:implantology theory (contraflow of menstrual bleeding,lymph and vein sprinkle),celothel epithelial metaplasia theory,induction theory and so on. Now the contraflow of menstrual bleeding implantology theory has been generally accepted. But above mechanisms unaccountability the other endometriosis morbidity cause such as the exterior cavitas pelvis gastrointestinal tract,urinary tract and so on. Endometriosis although a innocence disease,is similar to malignant disease distant place aversion and energy for growth, such as absoluteness growth, neovascularity and the quantity decrease of apoptosis cell and so on.The most conspicuous characteristic is have cell erosiveness, can part and distant place aversion,and infiltrate and damage other tissue,also to suffer the control of tumor metastasis.Recently a lot of studies have shown that anti-metastasis gene RECK and KISS-1 have an important contribution in many malignant tumor. RECK is a new anti-metastasis gene for the past few years discovered. RECK in many kinds of tissue of the health human body is express,in the tumor tissue is low express.KISS-1 polypeptide can obviously abaissement the chemistry tropism and infestation for the tumour cell, and its confine the function of the immigration and movements for the tumour cell. KISS-1 were expressed in the normal heart,liver,lung,musculi skeleti,nephridian tissue,and abdominal salivary gland,in the placenta and brain were high expressed.KISS-1 gene in mankind tumor metastasis have depressant effect,and to regulate accretion signal of the cancer cell.KISS-1 have potential effective to regulate the biology behavior. Many evidences had demonstrated that KISS-1 is lower express in EMT and play an important effect in the EMT development.Another report that RECK KISS-1 and MMP-2 have associativity. Some reference reported that the drop of the expression and activity of RECK play an important role in pathogenesis of EMT. So to investigate the expression of RECK KISS-1 and MMP-2 in endometriosis will have a very important meaning for the pathogeny of the EMT.ResearchThe purpose is to explore the relationship of RECK,KISS-1 and MMP-2 in EMT and to supply an new consideration for treatment and preservation of EMT.Materials and MethodsInpatients with endometriosis (n=30) accepted operation and the specimen collect at gynecological department of the Third affiliated Hospital of ZhengZhou University from july in 2008 to april in 2009,in which there were 16 cases in proliferate phase and 14 cases in secretary phase.Their endometrium tissues were assigned into two groups,the eutopic endometrium group and the ectopic endometrium group. The controls were the normal endometrium tissues of inpatients (n= 30) with uterine malformation for operations at the same time.The normal endomertium were investigated as the normal endomertium group. All the patients were collected at random. Their difference in age,weight and menstrual cycle has not statistically significant. All patients had no physical and surgical disease and had not accepted the hormone therapy six months before operations. The study used the Biosens Digital System to exam the average gray value of production of RECK KISS-1 and MMP-2.SPSS 16.0 was performed to analysis the study date. All the date was express as x±s. Means among several groups was compared by One-Way ANOVA and Means of every group was compared by LSD-T Test. Means between two groups was compared by t test. Correlations between groups were analysised by Pearson. Statistical significance was defined asα=0.05. Results1 The expression of RECK in eutopic and ectopic endometrium group was significantly lower than that in normal endometrium group (P<0.05). No significant difference was found in the expression of RECK between the proliferative phase and the secretory phase in eutopic Group and normal endometrium Group (P>0.05).2 The expression of KISS-1 in eutopic and ectopic endometrium group was significantly lower than that in normal endometrium group (P<0.05). The expression of KISS-1 was significantly lower in the proliferative phase than the secretory phase in normal endometrium Group (P<0.05). However, no significant difference was found in the expression of KISS-1 between the proliferative phase and the secretory phase in eutopic Group (P>0.05).3 The expression of MMP-2 in eutopic and ectopic endometrium group was significantly higher than that in normal endometrium group (P< 0.05). The expression of MMP-2 was significantly lower in the proliferative phase than the secretory phase in normal endometrium Group (P<0.05). However,no significant difference was found in the expression of MMP-2 between the proliferative phase and the secretory phase in eutopic Group of EMT (P>0.05).4 In entopic and entopic endometrial, the expression of RECK and MMP-2. was minus correlation(P< 0.05),the expression of KISS-1 and MMP-2.was minus correlation(P<0.05),the expression of RECK and KISS-1 was not correlation(P> 0.05).Conclusions1 The lower expression of RECK and KISS-1 and the higher expression of MMP-2 in endometriosis might play an important role in the genesis of endometriosis.2 The changes of RECK KISS-1 and MMP-2 in eutopic endometrum are associated with them in ectopic.It suggest that the apoptosis characteristic of eutopic endometrum of endometriosis might have changed. 3 The high correlation of RECK KISS-1 and MMP-2suggested that they may co participate in the generating of endometriosis.