Expression Of BFGF And PDGF-A In Choroidal Tear Trauma

Objective: Established animal mode of choroidal tear trauma in rabbits. To observe the changes of the expression of bFGF and PDGF-A in choroid, retina and vitreous at different time points after injury. Moreover, exploring the property of bFGF and PDGF-A at tissue repairing, to provide the new theroretical basis for clinical therapy.Methods: 40 adult rabbits were divided into 5 groups randomly, including normal group and experimental groups(3d, 7d, 14d, 28d after trauma). Among every group experiental eye was determined by parity method. Established animal model of choroidal tear trauma. Clinical symptoms were observed with ophthalmoscope and B-ultrasound at different times points. The morphologic changes of choroid and retina were observed by hematoxylin(HE) stain at light microscope. The expression of bFGF and PDGF in retina, choroid and vitreous were detected by SP immune histochemistry and ELISA assay separatly.Results: The expression of bFGF and PDGF-A increased at choroid, retina and vitreous after injury. The average optical density of bFGF and PDGF-A at choroid and retina and the mean OD value at vitreous both increased after injury at first and then decreased, and the peak period occurred at 7d to 14d after trauma. There were significant difference at normal group and experimental groups (P<0.05). There were no significant difference of 7d compared with 14d (P>0.05).Conclusion:â‘ bFGF and PDGF-A are important factors involved in the repair of choroid tear trauma and promote choroid fibrosis, scarring after trauma.â‘¡The peak period of the expression of bFGF and PDGF-A happened at 7d to 14d after trauma, during which cells were proliferating actively.â‘¢This reaserch provide the reference value to the clinical therepy.