Association Of CDH1 Promote Polymorphism (-347G�'GA) To Sporadic Colorectal Cancer

Objective: To investigate the association of－347G→GA polymorphism locatedin the CDH1 gene promoter region with risk of developing colorectal cancer(CRC)in China.Methods: The case-control study was designed, based on the population ofcolorectal cancer in China and healthy normal controls who have no tumor orinherited disease history. Polymerase chain reaction-restriction fragment lengthpolymorphism(PCR-RFLP) was used to genotype the variant, andimmunohistochemical staining was performed to measure the expression of Ecadherinin different allele cases including CRC patients and normal controls..Results: The GA-allele(G/GA heterozygous and GA/GA homozygous)didn'tincrease the risk of CRC compared with the G-allele(OR=1.227, 95%CI=0.887-1.698), but we found that the frequencies of the GA-allele were more high in poorlydifferentiated CRC patients than in normal controls (χ2-test, P=0.007), we alsoobserved that E-cadherin expression for poorly differentiated CRC patients waslower compared to moderately differentiated(t-test, P=0.001), and E-cadherinexpression for the GA-allele was also lower than the G-allele in CRC patients(t-test,P=0.018), but there was no statistically significant difference of E-cadherinexpression for the G-allele and the GA-allele in normal controls(t-test, P=0.292).Conclusion: These results suggest that－347G→GA promoter polymorphismof CDH1 was associated with CRC, but which might be a prognostic factor rather than a susceptive factor during the progression of CRC.