Molecular Epidemiology Of Rotavirus Among Children Under 5 Years Old Hospitalized For Diarrhea In Taiyuan

Objectives:To study the infected information, clinical symptom and nnmolecular epidemiological characteristics of HRV infection among hospitalized children with acute diarrhea under 5 years old in Taiyuan,from 2007 to 2008;to coreect the background data related with viral diarrhea;to provide references for prevention and treat of the disease.A total of 346 stools were collected from hospitalized children with acute diarrhea under 5 years old in Taiyuan between 2007,11 to 2008,10, Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR.These positive products were purified, extracted plasmid,then selected the speciemes which were confirmed by plasmid PCR test to be sequenced the nucleic acid.identify the genotype and genogroup of available strains.Resulits:A total of 346 stools were collected from hospitalized children with acute diarrhea under 5 years old in Taiyuan between 2007,11 to 2008,10. Of the 346 specimens, the percentage of samples with Rotavirus was 40.8%. Among 141 rotavirus positive samples, serotype G1 (42.6 %)was the predominant strain followed by G3(26.2%), G9(10.6%)and mixed-G infection(6.4 %).14.2% of strains remained to be non-typeable. P genotyping showed P[8](68.1%)was most common followed by P[4](0.7%), and non-typeable (24.1%). The most common combination of G and P was G1P[8] (31.9%),followed by G3P[8] (18.4%)and G9P[8] (7.1%). MOre than 95.7% of viral diarrhea patients under hospitalization occurred among children younger than 2 years.Conclusions:Rotavirus remains the most significant viral agent causing diarrhea hospitalization among children under the age of 5 years in Taiyuan. The most common children with rotavirus infections were younger than 2 years old. RV prevalence peaked fromSeptember to November. The predominant strains of rotavirus were G1 and P[8] in Taiyuan city. Information on the diversity and complexity of RV strains circulating in Taiyuan provides useful data for formulating vaccine policy and evaluating vaccine efficacy.