Study On The Rotavirus And Norovirus Diarrhea In Tianjin

Objective To analyze the infection status of virus diarrhea, clinical and epidemiological characteristics, disease burden, in order to provide scientific evidence for the development of prevention and control measures.Methods A total of1085patients with infectious diarrhea, who visited the selected5hospitals (Tianjin Children’s Hospital Nankai District Community Hospital, Xianshuigu Hospital, Tanggu Hospital and Baodi District Community Hospital) from2008to2009in Tianjin, were investigated, and their stool specimens were collected. Enzyme-linked immunosorbant assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect rotavirus, norovirus and their genotypes. Results Among1085infectious diarrhea patients, positive rotavirus antigen was detected in207patients, the detection rate was19.08%; positive norovirus antigen was detected in129patients, the detection rate was11.89%. Among790infectious diarrhea patients who were>5years, positive rotavirus antigen was detected in112patients, the detection rate was14.18%, positive norovirus antigen was detected in61patients, the detection rate was7.72%. Among295patients who were=5years, positive rotavirus antigen was detected in95patients, the detection rate was32.20%, positive norovirus antigen was detected in68patients, the detection rate was23.05%. The rotavirus detection rate and norovirus detection rate of the patients who were>5years is lower than that of the patients who were=5years. There was no significant difference in virus detection rates between men (28.66%) and women (28.67%). Among the rotavirus diarrhea patients who were>5years, genotype G3and P[8] were the most prevalent which accounted for29.46%and24.11%, whereas among the norovirus diarrhea patients, genotype GⅡ was the most prevalent which accounted for77.05%. Among the rotavirus diarrhea patients who were=5years, genotype G3and P[8] were the most prevalent which accounted for34.74%and26.32%, whereas among the norovirus diarrhea patients, genotype GⅡ was the most prevalent which accounted for83.82%. The detection rate of rotavirus were high during autumn and winter, while the detection rates of norovirus were no significant change throughout the year. The virus detection rates of the patients who were>5years are different among different districts, the suburban district is the lowest. There were more frequent diarrhea within24hours, more frequent fecal incontinence, fecal odor, vomiting, fever, loss of appetite, abdominal distension and borborygmus in the virus positive patients who were=5years than those who were>5years. Meanwhile more medical cost and more family burdens such as income damage, economic impact, entertainment activity plans abandoned, family members arguing and irritability were suffered in the virus positive patients who were=5years than those who were>5years.Conclusions The positive rates of rotavirus and norovirus were19.08%and11.89%in all diarrhea patients, and32.20%and23.05%in the patients aged=5years, respectively, which is a public health problem in Tianjin. Genotype G3and P[8] of rotavirus and genotype GⅡ of norovirus are prevalent. There were more frequent diarrhea within24hours in the virus positive patients who were=5years than those who were>5years, meanwhile more medical cost and more family burdens were suffered in the virus positive patients who were=5years than those who were>5years. So the virus diarrhea monitoring among infants and children should be strengthen in order to lessen the disease burden.