The Effect Of Liver Qi Yu Syndrome In The Metabolic Syndrome In The Basical And Clinical Research

BackgroundMetabolic syndrome is a variety of cardiovascular risk factors in the individual state within the assembly, it can not only increase the diabetes, the incidence of cardiovascular disease risk, but also increased the mortality rate of cardiovascular and cerebrovascular diseases, domestic and international concern in recent years hot spots.Objective1. Discussion of the metabolic syndrome liver qi Yu syndrome (MS) components of the metabolic effects in rats, liver qi Yu and MS rat liver X receptors (LXRs) to express the relationship between the Shugan Qi and the role of law in MS.2. Qi Yu pairs of MS patients to explore the metabolic effects of component and the card type and SAS, SDS Scale relationship.Methods1. Basical ResearchSixty-one healthy male SPF level just from the milk in spontaneously hypertensive rats (SHR) were randomly divided into normal diet control group (NC group,11), and high-fat diet group (FN group,50), FN group and the NC, respectively, by the cobalt-60 irradiated of the high fat diet and regular rodents fed. Feeding during the test once every 4 weeks body weight, body length, abdominal circumference, and rat tail artery blood pressure, in line with the basic features of metabolic syndrome in animal model after 12 hours fasting blood with an automatic biochemical analyzer fasting blood glucose, triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C). And the Caesarean section a portion of rat liver tissues were observed under light microscope pathological rats liver histopathological changes.Modeling the success of the above 33 MS rats were randomly divided into experimental group (18) and control group (15 rats). Experimental group, bound in metal shackles of bins, each restraint 30min, at intervals of 1 hour and tied a times, tied four times a day, until the emergence of the typical performance of qi Yu. Bound during the entire experimental group and control group in the same environment, do not be water and eat.Will be on the stage of modeling the success of 16 rats were randomly divided into MS qi qi Yu Yu MS control group (n=8, saline gavage), and qi Yu MS experimental group (n=8, fed Chaihushugan Powder); survival on the stage and the control group 12 rats were randomly divided into three MS simple MS control group (n=6, saline gavage), and pure MS experimental group (n =6, Chaihushugan Powder gavage).4 according lml/100g rats were fed one time each day continuously for 3 weeks.3 weeks after the blood-line inspection of relevant indicators, laparotomy the liver tissues by immunohistochemical staining, light microscope observation of four rats liver tissue expression of LXRs.2. Clinical ResearchEighty-six patients (male 42 cases, female 44 cases) MS patients according to traditional Chinese medicine clinic were divided into four qi stagnation (29 cases), phlegm Yongsheng (17 cases), blood stasis (19 cases), spleen deficiency (21 cases) four kinds of syndromes. All patients measured height, weight, waist circumference, hip circumference, blood pressure, blood glucose, blood lipids, blood coagulation in patients with other indicators and calculated body mass index, SAS, and SDS Scale standard score scale, to observe the target groups in different syndromes and the expression of Syndromes and SAS, SDS Scale relationship.Results1. After 14 weeks of feeding, the body weight, abdominal circumference, Lees index, blood pressure, fasting glucose, TC and LDL-C of 33 rats in the FN group compared with the control group were statistically different (p<0.05), successfully established animal model of MS. Control group, no significant edema in rat liver and fat changes; MS tissue lesions in rats with mild fatty liver change, moderate chronic inflammation, some showed focal necrosis of liver cells.2. After 20 days of modeling, experimental group,16 rats were compared with control group suffered from slow, behavioral retardation and markedly reduced, back hair and sparse brown non-Ze, tail trunk, covered with brown scales, weight loss (with compared with the control group p<0.01). In line with the typical performance in animal models of liver qi yu syndrome.3. Qi Yu MS experimental group and control group, blood pressure, fasting glucose, TG, TC, HDL-C was statistically significant (p<0.05); pure MS experimental group and control group, fasting glucose, TG, TC, HDL-C was statistically significant (p<0.05).4. Qi Yu MS rats in control group and experimental group LXRs results expressed strong positive and weak positive; pure MS control group and experimental group LXRs results expressed strong positive and weak positive.5.MS patient's age, systolic blood pressure, diastolic blood pressure, blood glucose, HDL in all Syndromes no significant difference between the two groups (P>0.05).6. Course of the diseaseLiver Qi stagnation group and phlegm Yongsheng Group, spleen and kidney deficiency group there are differences (P<0.05).7. Waist circumference values, TGLiver Qi stagnation, blood stasis group and the group, spleen and kidney deficiency group there are differences (P<0.05).8. Hips valuesLiver Qi stagnation, blood stasis group and the group, spleen and kidney deficiency group there are differences (P<0.05). Yongsheng phlegm and blood stasis group group, spleen and kidney deficiency group there are differences (P<0.05).9. HeightLiver Qi stagnation, blood stasis group and the group has significant difference (P<0.01), compared with the spleen and kidney deficiency are different (P<0.05).10. Weight Liver Qi stagnation, blood stasis group and the group, spleen and kidney deficiency group there was significant difference (P<0.01), phlegm Yongsheng group and the spleen and kidney deficiency group there are differences (P<0.05).11.Body mass indexLiver Qi stagnation, spleen and kidney deficiency group and the group has significant difference (P<0.01), phlegm Yongsheng group compared with the spleen and kidney deficiency are different (P<0.05).12. PT, FIBBleeding stopped within the group and the spleen and kidney deficiency group there was significant difference (P<0.01), and phlegm Yongsheng Group, liver Qi stagnation, a significant difference between groups (P<0.05).13. APPTBleeding stopped within a group and phlegm Yongsheng Group, spleen and kidney deficiency group significantly different (P<0.05).14. SAS and SDS standard scoreLiver Qi stagnation group and the other three groups had significant difference (P<0.01).ConclusionBe made in a cobalt-60 irradiation of the high fat fed SHR14 weeks, may be established animal model of MS. Liver qi Yu MS rats increased the metabolic components of the degree of MS in rat liver qi Yu affect the expression of LXRs, Shugan Qi method in rats the metabolic components of MS there is improvement.In this study, MS patients with a maximum distribution of qi stagnation. Different Syndromes of indicators between the two groups there are certain differences in patients with qi stagnation duration, height, weight, BMI, waist circumference, hip circumference, TG is higher than the rest of the card type groups, qi stagnation syndrome patients may have than the rest of the card type group worse prognosis. And qi stagnation syndrome in patients with SAS, SDS standardscore with the rest of the card type group were significantly different, which may be MS a new treatment method provides a new direction.