Xuebijing Injection Inhibits The Expression Of Tissue Factor Induced By LPS In Renal Microvascular Endothelial Cells And The Possible Mechanisms

ObjectTo investigate Xuebijing injection on the expression of tissue factor in renal micro-vascular endothelial cells(RMECs) induced by LPS in renal microvascular endothelial cells and to explore the possible mechanismsMethodsRMECs were isolated by means of the trypsin digestion technique, and cultured 2 to 4 passages after identification with FⅧ-related antigen immunofluorescence. Reverse transcription polymerase chain reaction (RT-PCR) was adopted to measure TF and ETmRNA expression. Immunofluorescence analysis was performed to evaluate the activation of NF-κB.ResultsCompared with the control group, obvious degeneration of rough endoplasmic reticulum and golgiosome were observed in the cells that cultured with 10mg/L LPS by electron microscope. LPS (1-100mg/L) increased TF and ETmRNA expression significantly (P<0.05). NF-κB apparently translocated from cytoplasm to the nucleus in the stimulated group; Xuebijing injection (12.5,25,50 mg/ml) significantly decreased TF and ETmRNA expression (P<0.05).Activation of NF-κB was not observed in the treated groups.ConclusionLPS increased TF and ETmRNA in a dose-dependent manner. Xuebijing injection inhibited the initial process of coagulation by decreasing the LPS-induced TFmRNA expression in microvascular endothelial cells.The possible mechanisms may be related to the reduction of ETmRNA expression and the inhibition the activation of NF-κB.