Expression And Relationship Of OPN And RAS In Human Endometrial Carcinoma

Endometrial carcinoma is a common neoplasm of the female genital tract, in contract to cervical cancer, it's incidence rate is stepping up constantly and is seriously threading women's health and has been one of the focus of the neoplasm research. It is molecular and pathophysiocal mechanisms are still unclear and lack of effective early detection. Recently, it has been found to contribute to tumorigenes is in several types of cancers. RAS genes are the first to be identified in human tumors, recent studies have shown that RAS participation in a variety of tumors and development. However the relationship between the two are rarely researched. In this study, we detected the expression of Osteopontin and RAS protein in endometrial carcinoma and normal control tissues, to evaluated the relationship between the expression of Osteopontin andRAS with endometrial carcinoma. So as to provide molecular evidence for endometrial carcinoma etiology and also to give a new way of diagnosis for endometrial carcinoma.MethodsThe SABC immunohistochemical method was used to detect the expressions of OPN and RAS proteins in 45 cases of endometrial cancer,28 cases of atypical endometrial hyperplasia and 18 cases of normal endometrial tissue. Every batch of staining are compared with HCC samples for positive-control, and replacing parmingen with control SPSS 16.0 software was employed to analyze all data, the data factors with Indepent sample Dunnett T-test and Anlysis of variance; And the relationship of the two factors was analyze by spearman rand correlation test.p<0.05 with significance.Results1. OPN was mainly stained in cytoplasm. The expressions of OPN in the tussues of endometrial carcinoma and atypical endometrial hyperplasia were significantly higher than that in normal endometrial tissues. The expressions of OPN correlated with the histopathological grading(G1 vs G2 Differentiated, P<0.05;G2 Differentiated vs G3 Differentiated, P<0.05), surgical pathological stage(P<0.05)and myometrial invasion(P<0.05), but no significant correlation with lymph node metastasis(P>0.05).2. RAS was mainly stained in cytoplasm. The expressions of RAS in the tussues of endometrial carcinoma and atypical endometrial hyperplasia were significantly higher than that in normal endometrial tissues. The expressions of RAS correlated with myometrial invasion(P<0.05) and lymph node metastasis(P<0.05),but no significant correlation with histopathological grading(P>0.05) or surgical pathological stage(P>0.05).3. There was significant positive correlation between OPN and RAS in endometrial carcinoma (r=0.3, P<0.05)Conclusion1. The expression of OPN and RAS were obviously stronger in endometrial carcinoma than in the normal control tissues, show that OPN and RAS may play a important role in the occurrounce of the endometrial carcinoma.2. The expression of OPN and RAS had relationship with myometrial invasion and,show that the expression of OPN and RAS had relation with the maligmnt degree of endometrial carcinoma.3. The expression of OPN and RAS had close correlation, indicated that OPN and RAS together play important role during the occurrence of endometrial carcinoma.