Effect Of Shugan Jieyu Yin On The Behavior And Sialic Acid Level In Serum Of Depression Model Rat

Depression is a kind of affective disorder diseases,with mood disorders,spiritual movement slow,Slowness of thinking and self-evaluation reduce as the main future. The patients usually represent bad mood, depressed, and pessimism, humble, to blame himself, and severe insomnia, anxiety, forgetful, even suicide. Also may be accompanied by weight, sex and pain subsided and other symptoms. The world health organization indicated that at present the world about 3.4 billion people suffering from depression, and the number is increasing year by year. By 2020, depression will become the second-largest after cardiovascular disease, which has become a very popular in this century. But its pathogenesis is very intricacy, which has not been completely stated, and there is no satisfaction in treatment. Therefore, the pathogenesis of depression and effective drugs in medical research become the hot issues. The chinese medicine treatment depression have good effects, and in security and reliability has a certain advantage. Therefore, the research of antidepressant single Chinese herbal medicine and TCM mixture has become a hot topic in China. Professor Youtian Li developed Chinese herbal medicine named Shugan Jieyuyin which is derived from the well known"Shugan powder"and"Xiaoyao powder". SGJYY has been applied to clinical treatment of depression, and achieved good results. Most patients` anxiety, insomnia, depression and other depressive symptoms are significantly improved after taking SGJYY. This topic is to study the effect of Shugan Jieyu Yin on the behavior and sialic acid level in serum of depression model rat.Methods: Choosing 48 clean grade healthy wistar rats, which its weight between 180g and 200g. They were fed one week to adapt to the environment, monitoring and recording body weight, sugar water consumption, level of activity scores and scores of vertical movement. Then, 48 rats were randomly divided into 6 groups, namely control group, model group, SGJYY2 g/ml, 4g/ml, 8g/ml dose group, fluoxetine group. Supporting the control group normal diet, do not give any stimulation; other groups were given 21 days solitary support and chronic unpredictable mild stress (including foot shock, forced swim, tail clip, tail suspension, day and night reversed, fasting, ban water, etc.) to construction depression model rats. During the modeling process we medicine the rats by gavage in the same time. The model control group received the same volume of saline; SGJYY small, medium and high dose groups were given SGJYY 2g/ml, 4g/ml, 8g/ml, 2ml each 1 time per day; fluoxetine group under the "man and animals according to body surface area equivalent dose conversion ratio formula" to calculate the daily dose in rats 0.36 mg, fluoxetine 20 mg will dissolve in 110 ml normal saline in, mix delivery 2 ml, per day. When the experiment to 7 days, 14 day and 21 days to monitor body weight of rats, swimming immobility time, the number of horizontal movement and vertical movement and sucrose consumption in each group. After 21 days medicine, we took blood from rat heart. We Injected chloral hydrate to anesthetize rats. The left hand fixed rats, using left hand index finger to touch the heart beat in the left intercostal 3-4, and right hand puncture the strongest division cardiac by syringe, blood 1-2 ml. 2500 rpm, centrifuge 5 min, serum was isolated, sub-put -70℃refrigerator after loading frozen. Through colorimetric method determine the level of saliva acid in rat. Observe the level of serum SA, and SGJYY effects on serum SA.Results: After 21 days alone to support and chronic unpredictable mild stress, the model control group, weight gain slowly, behavior scores and sugar water consumption also decreased significantly (compared with the control group, P<0.05), and rat performance is easily scared, reduced activities, dull coat, indicating that it is a successful rat model of depression. Positive drug fluoxetine group, SGJYY 4 g/ml, 8 g/ml group can significantly against the weight slow growth of rats in chronic stress model of depression (compared with the model group, P<0.05), compared with the control group there is no significant difference (P>0.05); can significantly against prolonged swimming inactive time (compared with model group, P <0.05), compared with the control group there is no significant difference (P>0.05); can significantly against decrease in the number of horizontal movement and vertical movement of depression model rats, and against decrease in sugar consumption (compared with model group, P <0.05), compared with the control group there is no significant difference (P>0.05). Fluoxetine serum SA concentration (compared with the control group, P <0.05), while the SGJYY2 g/ml, 4 g/ml, 8 g/ml dose levels of serum SA compared with the control group without differences (P>0.05).Conclusion: by monitoring body weight, swimming immobility time, the number of horizontal activity, vertical activity and number of sugar consumption on SGJYY pharmacodynamics observed, suggesting SGJYY significant antidepressant effects. And does not cause depression model SGJYY serum SA content increased, indicating the SGJYY more secure than fluoxetine.In summary, this study confirms SGJYY has a good antidepressant effect, and more safety than fluoxetine. It is scientific theoretical basis of clinical applications of SGJYY.