Study On The Significance Of Expression Of PDGF And Stat3 In Adenomyosis

Objective:To investigate the expression of platelet-derived growth factor (PDGF),STAT3 in adenomyosis eutopic and ectopic endometrial tissues and their effect in adenomyosis, in order to investigate the possible molecular mechanism of the pathopoiesis of adenomyosis and provide the theoretical evidence that contributes to uncover the new molecular targets of the the diagnosis and treatment of adenomyosis.Material and Methods:Tissue microarray and immunohistochemistry methods were employeed to study the expression of PDGF and STAT3 in 70 cases of adenomyosis specimens and 24 cases of CINIII (normal endometrium) specimens,to analyse the relationship between two indicators and clinicopathological factors. All data were processed by SPSS version 13.0 analysis software.Results:1. The result of factured TMA:The character of the recipient block was exquisite and had no splits. All chip cores were arranged orderly and clearly in the block except two chip cores were off. The chip cores had no shift and distort and obviously rugate in slides when they cut from block. They were answered for the request of research.2. The expression of PDGF was significantly higher in eutopic and ectopic endometrium of adenomyosis than normal endometrium (P< 0.05), however, no significant difference was found between adenomyosis endometrium and adenomyoma endometrium(P> 0.05).3. The expression of STAT3 was significantly higher in eutopic and ectopic endometrium of adenomyosis than normal endometrium (P< 0.05), however, no significant difference was found between adenomyosis endometrium and adenomyoma endometrium(P>0.05).4. The expression of PDGF and STAT3 in adenomyosis was correlated (r=0.274). The expression of them was not correlated with patient's age, dysmenorrhea, uterine size and menstrual flow.Conclusion: The expression of PDGF and STAT3 were higher in the endometrium of adenomyosis samples than normal endometrium, which suggested that PDGF and STAT3 are closely related to the pathopoiesis of human adenomyosis.