Study On The Biological Significance Of Expression Of Stat3 And Ki-67 In Adenomyosis Tissue Microarray And Its Relative Molecular Mechanism

Objective:To make clear the roles which Stat3 and Ki-67 played in occurrence,development of Adenomyosis and probable molecular mechanism of it, we intended to detect the expression of it in a Adenomyosis tissue microarray.We hope to supply a theoretical evidence for diagnosis,therapy and judgment of prognosis.Material and Methods:We applied tissue microarray technique and immunohistochemical method to detect Stat3 and Ki-67 protein expression and analyzed the relationships of them in an Adenomyosis tissue microarray containing Adenomyosis specimen and hysteromyoma specimen.All data were processed by SPSS version 13.0 analysis software.Results:1.The result of factured TMA:The character of our recipient block was exquisite and had no splits.The chip cores were arranged orderly and clearly in the block.The chip cores had no shift and distort and obviously rugate in slides when they cut from block.They were answered for the request of research.2.STAT3 proteins was respectively detected in 79.13%of all the 29 adenomyosis tissues and in 75.00%of all the 12 uterine adenomyoma tissues,which were higher than those of control group,and the differences were significant(P＜0.05).3.Ki-67 was respectively detected in 75.86%of all the 29 adenomyosis tissues and in 83.33%of all the 12 uterine adenomyoma tissues,which were higher than those of control group,and the differences were significant(P＜0.05).Conclusion:1.TMA,as a high and new technique,could be used on science research and clinicopathological diagnosis.It can save time and reagent.Low rate of experimental error is its important character.TMA will be a useful weapon for pathological scientists in science research.2.The expression of STAT3 was higher in Adenomyosis samples,in Hysteromyoma tissues its expression was 0,which suggested it involved in pathopoiesis of human Adenomyosis.3.The expression of Ki-67 was higher in Adenomyosis tissues,in Hysteromyoma tissues its expression was low,which suggested that Ki-67 is closely related to the proliferation of malignant cells and play an important role in mitotic of cells.