| Rohdea japonica (Thunb.) Roth.[Orontium japonicum thumb] has the function of clearing away heat and toxic material, strengthening heart and diuresis. Its indications are including sore throat, diphtheria, mumps, sore swollen drugs, mastitis, Snake bite, heart failure, edema, swelling, hemoptysis, hematemesis.The research of this paper originate from the book "Chinese medicine innovation-Pathogenesis of therapy":the medicine experience of Evergreen in heart failure in Yannian pill prescription. The book contains:Evergreen was invalid under the condition of 30g for decoction nor fried dry,it will also stop the heart in systole in used as powder. According to this book, we can get the following hypothesis:On the one hand the material basis of Rohdea japonica (Thunb.) Roth.may most contained in the water-soluble part than in the fat-soluble part, there may be some heat-sensitive material, easy to sublimation/evaporation material, we may get some information of this material basis by compare crude drug and fried dry drug. On the other hand, the Cure mechanism, the strong clinical side effects of powder may disprove the reliability of clinical experience, the cardiac mechanism of Everygreen may be more than one.This paper base on the research hypothesis,set up different analytic methods of Pharmacology and Pharmacodynatic and analyze different sample of Everygreen. The results are as follow:1,Rohdea japonica (Thunb.) Roth.'s component of an integrated pre-test and the establishment of tracking analysisThe system components of chemical pre-test results showed that Rohdea japonica (Thunb.) Roth. contains cardiac glycosides, polysaccharides, alkaloids, organic acids, which are chemical components with a variety of physiological activities and prospects, confirmed that in addition to cardiac e Rohdea japonica (Thunb.) Roth. glycoside addition there are other active substances. In this paper, We have established different parts of Rohdea japonica (Thunb.) Roth. TLC analysis method and HPLC-UV analysis method. By TLC analys in chloroform and ammonia in the presence of chloroform samples dilute bismuth potassium iodide can be the color of the component; TLC analysis showed that the water extracted samples there is no low-polarity and medium polarity dot.That means active ingredient of clinical experience of Rohdea japonica (Thunb.) Roth., including the broth does not contain alkaloids and terpenes low polarity. Detected on the HPLC peak and acid hydrolyzed samples were analyzed, the results show that HPLC-UV analysis method focuses on the cardiac glycosides were analyzed; Acid hydrolysis of samples of the new test have two obvious peaks. Analysis of these two peaks showed that the two peaks of UV-wavelength absorption and cardiac glycosides of different UV absorption characteristics, its structure and properties to be studied.2,Based on Component analysis method explore the clinical experienceThe paper on Rohdea japonica (Thunb.) Roth, for water, ethanol, chloroform extract were investigated, the result water-soluble extract more than 47 times of chloroform's, the clinical experience in Rohdea japonica (Thunb.) Roth. than the proportion of fat-soluble substances in water-soluble substances.Using the method of thin-layer chromatography to analyse the differences among the extracting solution of Rohdea japonica's crude sample, fried sample and pyrolysate will get the result that the crude sample and the fried sample appear a new spot. It's proved that there are something temperature sensitively in Rohdea japonica.Analyzing the different degree samples of the fried Rohdea japonica by HPLC-UV, the fingerprints are obviously different among them:at the location with 10.8 min, part of existing peak were not appear.It's also proved that there are something temperature-sensitively in Rohdea japonica. At the same time, it's also proved that cardiac glycosides is the effective components according to TCM clinical experience.This paper established the GC-MS dentification method of Rohdea japonica's pyrolysate. The results showed that there are something differences between the GC-MS and TLC analysis method, and their results can complement each other, but using the method of GC-MS will not be measured the existence of nitrogen element and the reason will be investigated further. 3,Exploration of "3g human heart can stop in systolic"ECG test showed that group of Rohdea japonica (Thunb.) Roth, powder group and decoction amplitude ECG are similar, but the soup powder dose equivalent decoction dose of 1/4 times, suggesting that Rohdea japonica (Thunb.) Roth. powder from the other components may exist in cardiac function; Ait powder and decoction of a relatively strong cardiac function, the powder and decoction on myocardial injury should be quite, but the result is higher mortality decoction, a shorter survival time after administration. Based on the experimental results suggest that treatment of heart failure Rohdea japonica (Thunb.) Roth, power may include the protection of myocardial function.4,Explore Rohdea japonica (Thunb.) Roth. non-cardiac glycoside positive inotropic effectPaper established the mouse PDE3 activity assay and used it to study the different samples of Rohdea japonica (Thunb.) Roth. effect on mice PDE3 enzyme. The results showed that Rohdea japonica (Thunb.) Roth. can inhibite normal mice PDE3, and further evidence of Rohdea japonica (Thunb.) Roth. may have other cardiac substances.This paper explored through clinical experience confirmed Rohdea japonica (Thunb.) Roth.:1) Rohdea japonica (Thunb.) Roth. clinical experience in addition to active ingredients cardiac glycosides, the alkaloids and decoction of the ingredients are not included in the above also may be an important component of clinical; 2) Rohdea japonica (Thunb.) Roth. pharmacology studies confirmed that chemicals may exist in the Rohdea japonica (Thunb.) Roth. power and the composition or the presence of myocardial protection of coronary artery expansion elements, while the strong presence of other cardiac components.This paper confirms the clinical experience of a certain science, but Rohdea japonica (Thunb.) Roth. "3 grams of contraction to cause cardiac arrest in the" experience at the end of the normal experimental animals and reflect the clinical experience of science continue to be fine.
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