| ObjectiveResearch Xiao-ke Wan to explore GK rats blood sugar and left ventricular function and blood to the myocardium was poorly organized and hunan, and the preliminary analysis Xiao-ke Wan for gk rats myocardium protection mechanisms.Methods1. To only 42 gk rats in blood sugar and the weight will be randomly divided into six groups of animals in a balanced and positive set of model, Xiao-ke Wan high, medium and low dose Xiao-ke Wans emerging out of the group in group and contrast,6-8 weeks male wistar normal the seven blank group, a total of seven groups, each group of seven.2. Xiao-ke Wan high,medium,low dose and pure Chinese medicine group were given Xiao-ke Wan 2800mg/kg,1400mg/kg,700mg/kg and 2800mg/kg;positive group received gliclazide 10 mg/kg; once a day, Continuous delivery at 12 weeks, control group and model control group were given the same amount of distilled water.3.Serum free fatty acids by, glycosylated hemoglobin, serum NO, nitric oxide synthase, serum insulin of the concentration of myocardial cells, AngⅡ, TGF-β1 The expression levels of several important biochemical factor in diabetic cardiomyopathy, the development of the role of Determine the extent of myocardial fibrosis; by echocardiography, cardiac histopathology, TUNEL method for the determination of myocardial apoptosis index Xiao-ke Wan in the GK rat cardiac lesions in the course of protection from its effect on several important biochemical factor expression levels.Result1.The weekend of 12, normal groups and drug groups, the model value of your stomach is empty and blood sugar and content of serum FFA,GHbAlc a significant increase, and weight and the Ins in reduced, has significant differences statistics (p<0.05). Model of serum NO concentration and the NOS activity clearly higher than the blank section (p<0.05). In Xiao-ke Wan high and medium doses group, the content of NO and NOS are activity significantly lower than the models (p<0.05).2.Experiment of 12 weeks, with normal group compared with normal, of the model rats's heart rate, LVSP, the+dp/dt,-dp/dt are down significantly (p<0.05), significant increases LVEDP (p<0.05); with a model of all drug in drug around the+dp/dt are able to significantly increase; Xiao-ke Wan high dose in the group and welcome the most significant reduction of LVEDP (p<0.05); have a significant increase in expect positive group LVSP role (p<0.05), Xiao-ke Wans, the-dp/dt the dose of a marked increase (p< 0.05), Xiao-ke Wan high dose is one of the high increasing.3.weekend of 12, Model sets of the myocardium ace activity and ii, ang tgf-beta an expression of a significant increase in normal (p< 0.01);with a model team in high doses Xiao-ke Wan, and group activities have significantly reduced ace(p< 0.01). With a model team in high doses Xiao-ke Wan, and group activities have significantly reduced ace(p< 0.01).The group of gliclazide, Xiao-ke Wan low the group and emerging out of the group with no discernible differences in the model team, Xiao-ke Wan high dose group ii and group in ang model has greatly decreased compared (p< 0.01), others are not see obvious differences.4. Experiment of 12 weeks, the experiment on rats model of the myocardium was poorly microscopic pathological changes, in the mitochondria, mitochondria internal abnormal go or not break, and empty, mitochondria part of the oars into the net, mitochondria, and muscle fibers Z blurred.the line for medicine and models of the group, the microscopic myocardium show pathological changes have different degree of improvement, with high dose Xiao-ke Wan of the myocardium microscopic pathological changes, and the myocardium was poorly cells inflate the cell in the normal, muscle fibers are arranged in neat silk, Z line clear, Mitochondria of iliac crest to underdevelopment and only a few of the mitochondria come to minor empty.Conclusion1. GK rat model by using Xiao-ke Wan was pharmacodynamics, the results show that the Xiao-ke Wan obvious myocardial protection.2. Xiao-ke Wans can be reduced GK rats serum and the myocardium no substantial increase and to curb excessive nos, perhaps by the way for the protection.3. Xiao-ke Wans GK is against the rats myocardium the apoptosis cells, in order to improve the level of the myocardium damage4. Xiao-ke Wans may by regulation GK rats ang ii of the myocardium was poorly organized and TGF-β1, the protection of the myocardium was poorly organized.
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