| Diabetic cardiomyopathy is a specific disease in absence of discernible coronary artery and characterized by long disease process , serious impairment and heart failure easily caused in late phase. The main manifestations are dysfunction of systole and diastole. The obvious pathological characteristics are hypertrophy and interstitial fibrosis. For its complicated mechanisms, the study about the diabetic cardiomyopathy is not clear. In this study, the diabetes models were made by 40 male SD rats with single injection of streptozotocin (50mg/μl) in tail vein and through 3 month progression the heart muscle will be impaired. 10 rats were injected citrate buffer solution in the tail vein as the control. In the sequential three month, the levels of NO and NOS in the rat serum, the homogenate of the heart muscle and the content of AngⅡ in the rat plasma and homogenate of heart muscle were assessed one time every month and were analysed . By the method of immuochemistry the expression of TGF-β1 in the ventricular cells were detected as the index to evaluate the degree of fibrosis. The method of TUNEL was used to measure the cardiomyocyte apoptosis index to explore its importance in process of diabetic cardiomyopathy.The results: 1. The weights of diabetic rats were apparently lower than those before the diabetic model was built (p<0.01), and the increase of weights in diabetic rats within three month were less than those in normal group.2. Compared with the control group, the amounts of NO and total NOS in the diabetic rats serum increased in one month and much higher in two and three month (p<0.01). There was no significant change of induced NOS in the rat's serum. The values of NO in the diabetic cardiac myocytes were much higher in the three-month group (p<0.01) and were not different in the first two month groups from the normal group. As compare with the normal group, the induced NOS in rats homogenate was much higher...
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