Preliminary Study Of The Effect Of Total Glucosides Of Paeonia (TGP) On T Cell Subset And Cytokines Of Th1 And Th2 In Patienes With Psoriasis Vulgaris

Objectives:To observe the changes of T cell subsets and Cytokine of Thl and Th2 to study the effects of total glucosides of paeonia (TGP) on T cell subset function andCytokine of Thl and Th2 in patients with Psoriasis vulgaris and to explore the feasibility of TGP as one of future clinical therapy of psoriasis vulgaris.Methods:The peripheral blood mononuclear cells (PBMCs) from 30 Psoriasis vulgaris were stimulated with phytohemagalutinin (PHA)(psoriasis-PHA group)or PHA together with TGP (psoriasis-TGP-PHA group)and were then cultured in vitro for 48 hrs. The samples from 30 healthys timulated with PHA were used as the Control group. The levels ofinterferon-y(IFN-y)and interleukin-4(IL-4)in the supernatants of PBMCs were detected using ELISA. And then,the level of CD3+,CD4+and CD8+ were detected using Flow CytoMete (FCM).Results:The level of IFN-γ,CD4+ and the ratio of IL-4/ IFN-y,CD4+/CD8+in the psoriasis-PHA group were statistically lower than those in the Control group(P<0.01). The level of IFN-y,CD4+and the ratio of IL-4/IFN-γ,CD4/+CD8+ in the psoriasis-TGP-PHA group were markedly higher than those in the psoriasis-PHA group(P<0.01), but were significantly lower than those in the Control group(P<0.05). There were no differences in the level of IL-4,CD3+, CD8+among the three groups.Conclusions:Level of Th2 cytokine and CD4+ are lower, Th1/Th2 cytokines and CD4+/CD8+are in imbalance in Psoriasis vulgaris patients suggesting that it plays an important role in the pathogenesis of psoriasis. TGP could increase the production of Th2 cytokine and CD4+,and regulate the Th1/Th2 cytokines and CD4+/CD8+ in patients with psoriasis. In order to explore TGP as one of the feasibility in clinical treatment of psoriasis vulgaris in future.