Study On Differentially Distrusted C45Isoforms From T Cell Subsets Between Psoriasis Patients And Healthy Individuals

Psoriasis is a chronic inflammatory and autoimmune diseases, although there isnot yet clarified the exact mechanisms that trigger psoriasis occurred, but a largenumber of clinical and experimental studies have shown that T lymphocytes play animportant role in the development of psoriasis occurred. Several CD45isoforms, suchas CD45RA、CD45RB、CD45RC、CD45RO,can be generated by alternative splicingof exons4（A）,5（B） and6（C） leading to change in the extracellular domain of themolecule. Importantly, polymorphisms and mutations that affect CD45alternativesplicing, and thus isoform expression, have been associated with several humanautoimmune diseases[1-4]. In this study, patients with psoriasis and normal humanperipheral blood T lymphocytes as a research object, useing flow cytometry to detectthe expression levels of CD45isoforms,which are abnormal or not. The experimentshelp to understand the mechanism of action of psoriasis development from the level ofimmune molecules provide clues to explore the psoriasis pathogenesis and futureresearch on new therapeutic targets.Objective: Detection the expression level of T lymphocyte CD45isoformsbetween psoriasis patients and healthy controls peripheral blood, to assess itsrelationship with disease severity and duration, In order to clarify that CD45isoformsplay a important role in the pathogenesis of psoriasis to support significant statisticallydata. Clarify the perspective of immunology Peripheral T lymphocyte subsets antigenexpression play a role in the pathogenesis of psoriasis, to provide science and effectiveimmunological strategy for psoriasis prevention.Methods: Select confirmed30cases of psoriasis patients as research subjects, and30cases of healthy people as controls, using direct double fluorescent staining by flow cytometry （Flow cytometry, FCM） to detecte the expression level of CD45isoforms: ofCD45RA, CD45RO, CD45RC, which are on peripheral blood T-cell surfaceResults:1. The analysis of CD45RC expression on human peripheral blood CD4T cells by flow cytometry revealed an heterogeneous expression, allowing the definitionof different subsets: CD45RC^highand CD45RClow. In contrast, the percentage ofCD45RCHighCD4+T cells in peripheral blood of patients with psoriasis significantlyhigher than normal, the difference was statistically significant （P <0.05）;but not CD8+T cell （P>0.05）.2. The expression of CD45RC⁺CD4⁺T in cells peripheral blood of psoriaticpatients is significantly higher than nomal（P <0.05）; but not CD45RC+CD8+T cells.3. The percentage of CD45RC⁺CD4⁺T peripheral blood of psoriasis was notcorrelation with PASI and disease duration.4. The analysis of CD45RO expression on human peripheral blood T cells by flowcytometry revealed an heterogeneous expression, allowing the definition of differentsubsets: CD45RD⁺and CD45RD^-T cell.5. The expression of CD45RD⁺CD4⁺T in cells peripheral blood of patients withmoderate-to-severe psoriasis is significantly higher than nomal subjects （P <0.05）;which is correlation with PASI,but not disease duration.6. The analysis of CD45RA expression on human peripheral blood CD4⁺T cells byflow cytometry revealed an heterogeneous expression, allowing the definition ofdifferent subsets: CD45RA⁺and CD45RA^-The expression of CD45RA levels of T cellin peripheral blood of patients with psoriasis compared with normal has no meaning.Conclusion:1. CD45RC⁺CD4⁺T lymphocytes in the peripheral blood ofpsoriasis patients with a relatively high expression, which was not correlated withPASI、disease duration of psoriasis patients.2. The expression of CD45RD⁺CD4⁺T incells peripheral blood of patients with moderate-to-severe psoriasis is significantlyhigher than healthy controls.And it correlated with PASI,but not disease duration.3. There are not abnormal expressions of CD45RA in peripheral blood T cells ofpsoriasis.