| IntroductionGastrointestinal cancer is one of the common malignant tumors in China, often leading to poor prognosis of patients and serious hazard to life and health. The transient receptor potential (TRP) ion channel family is a kind of potential-independent cation channel that extensively expresses in many organisms, tissues and cells. TRP channels are of great importance for a large variety of physiologic functions including muscle contraction, transmitter release, cell proliferation, cell differentiation, genetic transcription, apoptosis and angiogenesis. As a member of the TRPM (transient receptor potential Melastatin) subfamily, the TRPM7 channels are permeable to Mg2+ and Ca2+. It has been reported that the TRPM7 cation channels support multiple cellular and physiological functions, including cellular Mg2+homeostasis, intestinal pacemaking, cell viability and growth, synaptic transmission, cell adhesion, and its roles in cancers provoke people's interests. Recently, researchers have found that the TRPM7 channels have significant roles in the growth and proliferation of human mast cells, head and neck carcinoma cells and osteoblast-like cells. Kim et al. have suggested that the TRPM7 channels are critical for the survival of gastric adenocarcinoma cell lines. However, little is known about the presence and potential function of the TRPM7 channels in human gastric cancer. In this study, we examined the TRPM7 gene mRNA level in gastric cancer and their corresponding peritumor samples to approach the relationship of the TRPM7 and pathobiological behaviour of gastric cancer.Materials and MethodsMaterials1.48 fresh gastric cancer samples and their corresponding peritumor samples.2. Regents for total RNA extraction and RT-PCR. 3. Regents for Real-time PCRMethods1. Designing primers of the TRPM7 andβ-actin according to the gene sequences presented on NCBI.2. Total RNA of 48 fresh gastric cancer samples and their corresponding peritumor samples were extracted and transcripted into cDNA.3. Real-time PCR was used to test the expression of the TRPM7 mRNA in these samples.4. The correlation between the TRPM7 mRNA expression and pathobiological behaviour in gastric cancer was analyzed using SPSS 16.0.Results1. The TRPM7 mRNA was expressed in both gastric cancer samples and their corresponding peritumor samples. The levels of the TRPM7 mRNA of 48 cases of gastric cancer patients were detected, and 38 of which declined, accounting for 77.1% (37/48); while 11 increased, accounting for 22.9%(11/48). The expression levels of the TRPM7 mRNA in gastric cancer and the corresponding peritumor samples were 5.12±0.18 and 4.32±0.19, respectively. Comparing to the peritumor samples, the expression of the TRPM7 mRNA was markedly decreased in gastric cancer samples.2. In addition, the TRPM7 expression in gastric cancer samples with lymph node metastasis characteristics was obvious higher than that in non-metastatic gastric cancer samples.ConclusionThe repressed expression of the TRPM7 mRNA in gastric cancer samples demonstrated that the TRPM7 may promote the pathogenesis of gastric cancer and is likely to be associated with the metastasis of gastric cancer.
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