Detection Of HMLH1 And HPMS2 MRNA In Gastric Cancer Using Real-time Fluorescent Quantitative RT-PCR And It's Clinical Significance

AIM:To investigate the expression of DNA mismatch repair gene hMLH1/hPMS2 and their clinical significance in gastric cancer.METHODS:We detected the level of hMLH1/hPMS2 mRNA respectively in 40 cases of gastric cancer and 40 cancer-adjacent mucosa tissues simples of gastritis and 21cases gastritis tissues with real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The reference for normalization of the expression level that we used is the human glyceraldehydes-3-phosphatedehydrogenase (hGAPDH) gene.RESULTS:The level of hMLH1 mRNA in gastric cancer tissues was 7.23±11.91, in the corresponding cancer-adjacent mucosa tissues was 3.80±5.13, and in the chronic gastritis tissues was 2.01±1.25. The level of the hMLHl mRNA in gastric cancer was markedly higher than that in the other tissues (P<0.05).And in the corresponding cancer-adjacent mucosa tissues, the level of hMLH1 mRNA was significantly higher than that in the chronic gastric mucosa tissues(p<0.05). The level of hPMS2 mRNA in gastric cancer tissues was 0.43±0.35, in the corresponding cancer-adjacent mucosa tissues was 0.55±0.39, and in the chronic gastritis tissues was 0.32±0.15. The level of the hPMS2 mRNA both in gastric cancer tissues and in the corresponding cancer-adjacent mucosa tissues was markedly higher than that in the chronic gastric mucosa tissues (P<0.05). But in gastric cancer tissues, the level of hPMS2 mRNA was no difference compared with that in the corresponding cancer-adjacent mucosa tissues (P>0.05). The expression level of hMLH1/hPMS2 mRNA in gastric cancer has no visibly relationship with the size, the degree of infiltration of tumor and the metastasis of lymph node(P>0.05), except that expression level of the hMLHl mRNA was higher in gastric cancer with the metastasis of lymph node than that without the metastasis of lymph node(P<0.05).CONCLUSION:The expression level of hMLH1/hPMS2 gene in gastric cancer and in the corresponding cancer-adjacent mucosa were abnormal compared with that in the chronic gastritis. This abnormal transcription maybe related with gastric cancer, but maybe not involved in the development of gastric cancer.