Detection And Clinical Significance Of HPMS2 MRNA In Gastric Cancer Using Real-time Fluorescent Quantitative RT-PCR

AIM:To investigate the expression of DNA mismatches repair gene hPMS2 and its clinical significance in gastric cancer.METHODS:Real-Time fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR) was used to detect the level of hPMS2 mRNA respectively in 41 cases of gastric cancer and 37 cancer-adjacent mucosa samples of atrophic gastritis and 25 cases of chronic atrophic gastritis and 20 cases of chronic superficial gastritis.The human glyceraldehydes-3-phosphate dehydrogenase(hGAPDH) gene was used as a reference for normalization of the expression level.RESULTS:The expression levels of hPMS2 mRNA in gastric cancer,the corresponding cancer-adjacent mucosa,chronic atrophic gastritis and chronic superficial gastritis were 28.33±14.05,16.88±10.67,7.25±8.72 and 1.78±1.34,respectively.Significant differences were detected among the four groups(F=31.82,P=0.00).The expression level of hPMS2 mRNA was markedly higher in gastric cancer than in the other tissues(P＜0.01),and significantly higher in the corresponding cancer-adjacent mucosa than in nonneoplastic mucosa(P＜0.01),but no significant difference was detected between chronic atrophic gastritis and chronic superficial gastritis.The expression of hPMS2 in gastric cancer had no obviously relationship with the size,the degree of infiltration of tumor nor the metastasis of lymph node.CONCLUSION:The abnormal transcription of hPMS2 gene maybe related to gastric cancer,but not involved in the development of gastric cancer.