The Effect Of Tautine On The Neurotoxicity Induced By Manganese Chloride In Rat's Hippocampus

Objective:To study the effect of taurine on manganese chloride neuroto-xicity in rat's hippocampus in vivo.Methods:SD rats were divided into five groups after one week of observation:A:24w normal control, the group animals received daily of intraperitoneal(ip.) injections of sterile saline for 24 weeks. B:24w Tau treated group:First, rats were received ip. injection of MnCl2·4H2O 15mg.kg-1/d once a day for 12 weeks, then the rats received Tau 200mg/kg three times per week for 12 weeks. C:12w Mn treated group:rats were recievd the daily injection of Mn as Tau treated group for 12 weeks. D:12w normal control group:rats were received ip. injection of sterile saline as group A for 12 weeks. E:Tau preventive group:The Mn level of this group are the same as Mn's, the tau level are the same as group B,three times per week, the assayes remained for 12 weeks. At the end of the experiment, a period of 1 week morris water maze test(MWM) began immediately. End of the test, that is, the rats were killed, isolated rat hippocampus, were detected on the following indicators:electron microscopy of apoptosis in hippocampus, changes in organelles and ultrastructure; ROS kit on mitochondrial reactive oxygen species were measured; respectively WB, RT-PCR from the protein level of detection of Bcl-2 gene family (Bcl-2, Bax, Bcl-xl, Bak), apoptosis inducing factor AIF and ChAT in the hippocampus of the expression.Results:①MWM Show:escape latency of the overall downward trend. C group was significantly longer than E group (P<0.05), B group and A group of non-discrimination. But the A group and B group was higher than D group and E group (P<0.05).②manganese can induce the apoptosis of hippocampal neurons and cause damage to organelles, taurine could antagonize the neurotoxicity of manganese to improve the injury.③prevention and treatment of taurine reduced manganese can effectively stimulated mitochondrial reactive oxygen species increased.④manganese can raise Bax, Bak, AIF expression, reduced Bcl-2, Bcl-xl expression, and then exposed to manganese compared with taurine to taurine treatment to more effectively protect the hippocampus, inhibition of apoptosis factors in manganese this induction. However, some apoptotic factors by western blot and RT-PCR determination of the results not fully consistent with the results, pending further analysis.⑤ChAT manganese can cause reduced protein expression, taurine can attenuate the effects of manganese.Conclusion:Manganese may be through oxidative stress, induced apoptosis leads to nerve damage, by inhibiting the synthesis of Ach to reduce cholinergic neurons in excitatory synaptic transmission, the end result of learning and memory dysfunction.Taurine by oxidation, regulation of apoptotic factors and enhance the expression of antagonistic ChAT manganese toxicity.