A Study On The Correlation Between Plasmic Visfatin & Its Single Nucleotide Polymorp And The Lipid Metabolism Of Polycystic Ovarian Syndrome

ObjectivesTo detect the levels of plasmic visfatin and lipids of the patients with PCOS and assess their association; To investigate the availability of the single nucleotide polymorphism (SNP) of the visfatin gene locus rs11977021 among local PCOS patients and, evaluate the differences in the genotype distributions of the visfatin gene locus between PCOS patients and the control subjects; To speculate the etiology and pathogenesis of PCOS and its long dated complications.MethodsSelect 50 patients with PCOS and 28 control subjects; determine the waist hip ratio (WHR), body mass index (BMI) and plasmic lipid levels; determine the plasmic visfatin levels with enzyme-linked immunosorbent assay(ELISA); to analyze the genotype of the visfatin gene locus rs11977021, with the technology of Ligase Detection Reaction-Polymerase Chain Reaction (LDR-PCR).Results1. The contrasts of the plasmic visfatin levels: in terms of plasmic visfatin level, the PCOS group is higher than the control group (5.67±2.08ng/ml vs 4.86±1.26ng/ml, P<0.05); the obese control group is higher than the non-obese control group(5.48±2.03ng/ml vs 4.68±1.21ng/ml, P<0.05); and the plasmic visfatin levels between the remaining groups have no statistical differences.2. The contrasts of the plasmic lipids levels:①TC: the PCOS group is higher than the control group(4.63±0.76mmol/l vs 3.76±0.73mmol/l, P<0.05); the non-obese PCOS group is higher than the non-obese control group (4.80±0.70mmol/l vs 3.85±0.81mmol/l, P<0.05); the obese PCOS group is higher than the obese control group, (4.52±0.79mmol/l vs 3.50±0.40mmol/l, P<0.05);②TG: the PCOS group is higher than the control group (1.31±0.85mmol/l vs 0.80±0.43mmol/l, P<0.05); the non-obese PCOS group is higher than the non-obese control group(1.48±1.13mmol/l vs 0.83±0.42mmol/l, P<0.05) ;③HDL: the control group is higher than the PCOS group (1.52±0.21mmol/l vs 1.36±0.26mmol/l, P<0.05); and the plasmic lipids levels between the remaining groups have no statistical differences.3. There are positive correlations between the plasmic visfatin levels and BMI(r=0.500, P<0.01) &WHR(r=0.620, P<0.01); and a negative correlation between the plasmic visfatin levels and HDL (r=-0.760, P<0.01).4. Among the 3 genotypes in visfatin gene locus rs11977021, there are 14 C/Cs,12 T/Ts and 20 C/Ts respectively in the PCOS group and, 6 C/Cs, 1 T/T and 21 C/Ts respectively in the control group, and there are statistical differences between the constituent ratios of the genotypes at this locus.Conclusions1. There is a high visfatin level with PCOS patients.2. There is a lipid metabolism disorder with PCOS patients, and the visfatin level is correlated with the lipid metabolism.3. There exists a single nucleotide polymorphism (SNP) of the visfatin gene locus rs11977021 with local PCOS patients, and the genotype distributions of the visfatin gene locus rs11977021 differ between PCOS patients and the control subjects; the single nucleotide polymorphism (SNP) at this locus might be correlated with the pathogenesis of local PCOS .