| Background:Chronic Wounds are very common and difficult to be treated successfully in the clinical practice. Therefore they become one of the hot points of surgery research areas many years ago. The principal measure of treatment in western medicine is still through the intervention of debridement. But this measure has the shortcoming of improving trauma and suffering of patients first. The development of various growth factors and their clinical application proved to be effective, but with the disadvantages of unstable effects, less biological stability and side effects of heart disease and tumor generating possibilities. There are excellent effects of Traditional Chinese Medicine on chronic wound healing with the abundant experiences of several thousand years clinical practice, of which SHENG JI YU HONG Plaster is one of the classical topical medicine with excellent practical results. Object:To explore the mechanism of SHENG JI YU HONG Plaster on wound healing by evaluating its effects of ameliorating wound microcirculations.Methods:The first part of experiment is the animal experiment with 30 SD rats, half of which is for experiment group and the other half is for the control. As for model made, the round size of 1.8cm diameter of full thickness back skin was cut mechanically and covered with petrolatum gauzes, SHENG JI YU HONG plasters respectively. At day3, 5, 7 and 14 after model made, the wound surface healing rate, the amount of hydroxyproline, hemoglobin, the micro-vascular density (MVD) by HiCN, 722 spectroscopical luminosity and MPIAS2500 multicolor pathological graph analytical system. The second part is clinical research with 30 patients of chronic ulcers, half of which is for experiment group and the other half is for the control. The samples are granulation tissues in the center of wound. The intervention measures are the same as animal experiment, except for the samples received at day 3, 7 and 14.Results:1 The MVD of wound granulation tissues is higher with SHENG JI YU HONG Plaster intervention than that of petrolatum gauzes intervention with statistic differences (P<0.01), both in animal and clinical experiments.2 The amount of hemoglobin of wound granulation tissues is higher with SHENG JI YU HONG Plaster intervention than that of petrolatum gauzes intervention with statistic differences (P<0.05), both in animal and clinical experiments.3 The amount of hydroxyproline of wound granulation tissues is higher with SHENG JI YU HONG Plaster intervention than that of petrolatum gauzes intervention with statistic differences (P<0.01), both in animal and clinical experiments.4 The rate of wound surface healing is higher with SHENG JI YU HONG Plaster intervention than that of petrolatum gauzes intervention with statistic differences (P<0.01) Conclusions: 1 SHENG JI YU HONG Plaster can positively enhance the MVD, the amount of hemoglobin and hydroxyproline of mechanical wound in rat and chronic wound in patients.2 One of the mechanisms of SHENG JI YU HONG Plaster for improving wound healing maybe through ameliorating wound microcirculation.
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