Research Of Bovine Jugular Venous Treated With Decellularization And Polyepoxy Compound

Objective To Evaluation the Biomechanic property and anti-calcification properties of BJV treated with decellularization and polyepoxy compound. Study the feasibility of it as a new biomaterial substitution in cardiac surgery.Methods Each of twenty fresh bovine jugular venous was cut into 6 trial patches,which were randomly divided into six groups, which were fresh (group A) and treated by the techniques of polyepoxy compound (group B), decellularization(group C), decellularization and polyepoxy compound(group D) , decellularization and glutaraldehyde (group E), decellularization and dye-mediated photooxidation (group F) respectively.The morphologic properties of test specimen were evaluated by HE stain .The Biomechanic property of six groups, including elastic modulus, tensile strength, tensile stress at tensile strength, tensile strain at tensile strength were studied. One of the trial patches in each groups was implanted subcutaneously in the same SD rat (total 20 rats) . All trial rats were sacrificed 60 days after implantation, and the specimen were retrieved. The morphologic properties of test specimen were evaluated by HE stain.The tissue calcium content was determined by flame atomic absorption spectrophotometer. The tissue calcium salt distribution was evaluated by Von Kossa calcium salts stain , X-ray , spiral computed tomography and scanning of bone density.Results (1) The group D was close to fresh bovine jugular venous, The cell components in the group C to F were completely removed,the construction of matrix and the collagen fibers were maintained well. (2) The Biomechanic property manifests the elastic modulus and The tensile stress and tensile strain at tensile strength of group D are lower than group B and higher than group A (P<0.05) .The tensile strength of group D lower than group B and higher than group A, C and F (P<0.05) ,but there was no difference with group E.(3) the tissue structure of the test specimen in group D kept intact, the tissue degradation was slight.The cells mostly infiltrated in out layer and there was no sand-like nodus.The calcium content of group D was lower than the group B and was no difference with other groups.The Von Kossa calcium salts stain showed the tissue calcium salt distribution in the group D lower than the group A and B, slightly higher than group C and similar to group E and F. The calcium salts density in total of the trial patches in group D was significantly less than those of group B and similar to which of group E,F and slightly more than group A and C.Conclusion1,The technic of BJV treated with decellularization and polyepoxy compound is a effective way to fix biomaterial;2,Compared to treated with polyepoxy compound, it has proper Biomechanic property and more excellent anti-calcification properties.