Go Cell Combined With Photo-oxidation Technology To Deal With The Bovine Jugular Vein Conduit Anti-calcification Properties

Objective To study the anti-calcification properties of BJVC treated with dye-mediated photooxidation following decellularization in vivo.Methods Each of twenty fresh bovine jugular veins with a retained native valve procured from a slaughterhouse was cut into 4 trial patches with valve leaflet,which were randomly divided into four groups as group A, B, C and D, which were treated by the techniques of dye-mediated photooxidation (group A), glutaraldehyde (group B),decellularization (group C) and dye-mediated photooxidantion following decellularization (group D) respectively. One of the trial patches in each group was implanted subcutaneously in the same Wistar rat, total 20 animals. All trial rats were sacrificed 60 days after implantation, and the specimen were retrieved. The morphologic properties of test specimen were evaluated by HE stain and electron microscope.The tissue calcium content was determined by flame atomic absorption spectrophotometer. The tissue calcium salt distribution was evaluated by Von Kossa calcium salts stain,X-ray, spiral computed tomography, scanning of bone density.Results Compared with which in other groups, the test specimen in group D were soft and tenacious, its fibrous structure kept intact, the tissue degradation was slighter than which in goup C. The calcium salts density in total of the trial patches in group D was significantly less than those of group A and B, and was similar to which of group C. The calcium content of vessel wall in group D (6.08±4.39μg/mg) was significantly lower than those of group A (105.42±39.06μg/mg) and B(154.70±25.44μg/mg) (P<0.01), and was no difference with which of group C (5.75±3.90μg/mg) (P>0.05). The calcium content in the valvular leaflets in group D (3.34±2.29μg/mg) was lower than which of group B (23.35±10.75μg/mg) (P<0.01), and was no difference with those of group A(6.03±3.89μg/mg) and group C (4.22±2.30μg/mg) (P>0.05).Conclusion The technique of dye-mediated photooxidation following decellularization can effectively prevent the calcification of bovine jugular venous conduit in vivo .