Toxicological Evaluations Of The Bovine Jugular Vein Graft Treated By Glutaraldehyde/Octanol,Polyepoxy Compound And Dye-mediated Photooxidation

Objective: 1. To evaluate the mutagenic toxicity of the bovine jugular vein grafts (BJVG) which are treated by Glutaraldehyde/Octanol , Polyepoxy Compound (PC) and Dye-mediated Photooxidation by the micronucleus (MN) test, chromosomal aberration (CA) test and Ames test; 2. To evaluate the systemic toxicities of these biomaterials by the pyrogen test, acute toxicity test, subacute toxicity test and chronic toxicity test.Methods: 1. Mutagenic toxicity: (a). The micronucleus test: The extracts of the treated BJVG and the controls were injected into the abdominal cavities of the mice. The injection was repeated every 24hs and totally 4 times, then after 24hs of the last injection the Giemsa-stained slides of the femoral bone marrow were made. The MN rates of the polychromosome erythrocytes (PCE) were scored and analyzed by SPSS 12.0. (b). The chromosomal aberration test: The injection of the extracts and the controls was the same as the micronucleus test. The Giemsa-stained slides of the femoral bone marrows cells were made 4hs after colchicine had been injected into the abdominal cavities of the mice, by the process of centrifuge-hypotonic treatment-fixation-smear-stain. The CA rates of the bone marrow cells were scored and analyzed by SPSS 12.0. (c). The Ames test: The extracts and the controls were added to the nutrient mediums in which the Salmonella typhimurium were inoculated, with the S-9 system added or not. The numbers of the strains in each culture dish were scored after 48hs of culture.2. Systemic toxicity: (a). The pyrogen test: The temperature was measured 3 times before, the mean temperature calculated, and 6 times after the extracts were injected into the veins of the rabbits. The maximal and total increase of the temperature of each rabbit were calculated, (b). The acute toxicity test: The extracts and the controls were injected into the veins of the mice and the degrees of the toxical effects were observed 4hs, 24hs, 48hs and 72hs later. (c). The subacute toxicity test: The injection of the extracts and controls was the same as the acute toxicity test and repeated every another day. The blood routine test and...