| Objective: To study the expression of Id1 and Id3 in human non-small cell lung cancer (NSCLC), and to analyze its potential clinical significance.Methods: 60 paraffin-embedded specimens were collected and the specimens were got from the patients with NSCLC in the first affiliated hospital of Chongqing Medical University from 2004 to 2007. The cases were separately grouped according to the age, gender, pathological type of tumor, lymph node metastasis, etc. The pathological results were all confirmed by the department of pathology of Chongqing Medical University. Then, the expression of Id1 and Id3 were detected by immunohistochemical staining technique. The positive signals of Id1 and Id3 were the yellow or brown staining in intracytoplasm. We used the method of staining intensity×staining cells to analyze the results. We had done the contrast analysis to find out the reasons of the different expression of Id1 and Id3 in each group and the correlation analysis to find out the relationship of Id1 and Id3 in all NSCLC samples.Results: There were no Idl or Id3 expressions in normal lung tissue, Negative to weak expression of those could be observed in benign disease tissues. However, the expressions of Id1 and Id3 were obviously strong in NSCLC, which was significantly higher than that in benign disease (P<0.01). Furthermore, the over expressions of Id1 and Id3 significantly correlated with lymph node metastasis (P<0.05) ,and they were not related to pathological type, the age or the gender of the patients(P>0.05). The correlation analysis showed positive correlation of them.Conclusion: Idl and Id3 protein are overexpressed in NSCLC and the positive expression is correlated with lymph node metastasis. It suggested that Idl and Id3 are associated with tumorigenesis, invasion and metastasis in NSCLC. Idl and Id3 might be used as new markers of diagnosis and prognosis for NSCLC.This finding might put forward a new method for NSCLC treatment.
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