The Effects Of Yinchenchengqi Tang On Coagulation Of Severe Acute Pancreatitis In Rats

Objective: To explore the effect of Yinchenchengqi Tang on cruor function in severe acute pancreatitis (SAP) in rats. and provide a theoretical basis and new treatment ideas for the treatment of SAP of traditional Chinese medicine.Methods: Healthy SD 30 male rats were randomly divided into three groups: control group; model group; Chinese medicine treatment group.each group 10. Model-building steps:(1)anesthesia with 4% sodium pentobar bital in accordance with 1 ml / kg body weight by intraperitoneal injection of doses of narcotic. (2)anesthesia after the entry into force, fixed in rats in the surgery board, check supine position, abdominal rat hair cut, conven- tional disinfection Shop surgical towel ready.(3)from upper middle incision about 3 cm in the open under strict aseptic abdominal operation to find the junction of stomach and duodenum, and then down to find the opening cholangiopancreatography duodenum. (4)hepatic artery occlusion Explorer folder, No.4.5 needles will be up on the flat cholangiopancreato graphy subcapsular duodenal puncture opening line to the duct direction about 2-3mm, by 1 ml / kg body weight will be 4% sodium deoxycholate to retro- grade pancreatic duct injection of (0.2 ml / min), so that uniform uplift of the pancreatic duct from the needle, that needle into the hand of oppression of approximately 3-5 min, then remove the folder artery, abdominal closure, and at the same time to observe the beginning of time. control group was sham-operated group, model group by the pancreatic duct to the pancreatic tissue in rats by retrograde injection of 4% sodium deoxycholate method of severe acute pancreatitis in rats induced by severe acute pancreatitis (SAP), Chinese medicine treatment group and model group SAP produced the same rat model, the former model in 12h and 12 h after the model oral gavage Yinchenchengqi Tang liquid 2ml / g, the rats on the prothrombin time (PT) , activated partial thromboplastin time (APTT), fibrinogen (FIB) and international normalized ratio (INR) compared to each other.Results:(1)PT: mild extension of PT of the model group, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group has been to shorten the PT, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).(2)APTT: model group with mild APTT prolonged, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group, APTT has been shortened, the difference is not statistically significant (P> 0.05), there was no significant difference Chinese medicine treatment group compared with the control group (P> 0.05).(3)FIB: FIB of the model group was significantly higher, compared with the control group with a statistically significant difference (P <0.01), traditional Chinese medicine treatment group compared with the model group FIB decreased significantly (P <0.01), but still the control group increased (P <0.01).(4)INR: model group INR increased slightly, compared with the control group did not have statistically significant differences (P> 0.05), traditional Chinese medicine treatment group compared with the model group had no significant changes in INR, the difference is not statistically significant (P> 0.05), Chinese medicine treatment group compared with the control group there was no significant difference (P> 0.05).Conclusion: Yinchenchengqi Tang can effectively reduce severe acute pancreatitis in rat blood fibrinogen (FIB) content, to improve blood coagulation in rats with severe acute pancreatitis severity of dysfunction for clinical treatment of SAP to provide the theoretical basis and new ideas; severe acute pancreatitis in rats fibrinogen (FIB) was significantly increased, prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in the 24h does not significantly change; Yinchenchengqi Tang had no significant effect on rats with severe acute pancreatitis on the prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR) in a short period of time.