Studies On Granularity And HPLC Fingerprint Of Mulberry Leaves Ultra-fine Powder

Mulberry leaves is the dry leaf of Morus alba L.,which is a well-known Traditional Chinese Medicine named Sangye.The main constituents include saponin,flavonoid,alkaloid,polysaccharide etc,which have the character of lowing blood glucose,hypertensive,antihyperlipidemia,bacteriostasis and antivirus,so the material medical and functional food of Mulberry leaves will be a bright developing and studying future.In this research,we studied the microscopic characteristics of mulberry leaves ultra-fine powder.A typical microscopic characteristic was not observed. Which showed the cell walls broken up.We studied the Granularity of mulberry leaves ultra-fine powder by microscope,D₅₀ is 25.1μm. In this research,HPLC was used to test the content of Rutin.Chromatography conditions were Hypersil BDS-C₁₈(4.6mm×250mm,5μm)column.The mobile phase were Methyl and 0.5%H₃PO₄ water,the analyses performed by gradient elution,the rate of flow is 1.0ml/min,and detected at 360nm.The content of Rutin in the ultra-fine powder was between 0.10%and 0.49%.In this research,HPLC was used to develop fingerprint.Chromatography conditions were Hypersil BDS-C₁₈(4.6mm×250mm,5μm)column.The mobile phase were Methyl and 0.5%H₃PO₄ water,the analyses performed by gradient elution,the rate of flow is 1.0ml/min,and detected at 320nm.19 Mulberry leaves ultra-fine powders were detected,the resutlt showed 12 peaks were common in total;the dates analyzed by the "Computer Aided Similarity Evaluation" software,the relative degree between 0.69 and 0.99.