Study The Relationship Between The Expression Of PCNA, P53 And Helicobacter Pylori Infection And The Raised Erosive Gastritis With Spleen-Stomach Dampness-Heat Syndrome

Objective:To study the relationship between the expression of PCNA,p53 and Helicobacter pylori infection and the Raised Erosive Gastritis with spleen-stomach dampness heat syndrome.To study the relationship between TCM syndrome and PCNA,p53, Helicobacter pylori;meanwhile observe the difference of pathological and molecular biology among different syndromes.In order to assistant the treatment of the Helicobacter Pylori Raised Erosive Gastritis with integrated traditional Chinese and Western Medicine.Method:79 Raised Erosive Gastritis patients whoses syndromes meet with spleen stomach-dampness-heat(SSDH)and spleen stomach qi-deficiency(SSQD)accroding to TCM dialectics were selected as research subjects.There were 54 patients in spleen-stomach dampness-heat(SSDH) group,25 patients in spleen stomach qi-deficiency(SSQD)group.In SSDH group,there were 36 patients whoses HP is positive and 18 patients whoses Hp is negative.In SSQD group,there were 16 patients whoses Hp is positive and 9 patients whoses Hp is negative.There were 18 patients in control group.All subjects accepted gastroscopy examination.We got the samples from the gastric antrum,during the gastroscopy examination;and then the samples were used to do rapid urease test,detect the existence of Hp with histological stain method,do pathologic diagnosis and detect the expression of PCNA,p53 with immunohistochemistry.Results:1 The degree of atrophy,intestinal metaplasia in the SSDH group was much worse compared to it in the SSQD group(p＜0.05).In SSDH group,the result was the same when degree of atrophy,intestinal metaplasia in the Hp positive group and Hp negative group,but in the SSQD group,there were no difference between Hp positive group and Hp negative group about the degree of atrophy,intestinal metaplasia.2 The expression of p53 in Raised Erosive Gastritis with SSDH was enhanced and obviously different with in the SSQD group and in the control group(p＜0.01),In SSDH group,the result was the same when the expression of p53 in the Hp positive group and Hp negative group(p＜0.05).but in the SSQD group,there were no difference between Hp positive group and Hp negative group about The expression of p53(p＞0.05).3 The expression of PCNA in Raised Erosive Gastritis with SSDH group and SSQD group was enhanced and obviously different with in the control group(p＜0.01),.there were no difference between SSDH group and the SSQD group(p＞0.05).In SSDH group,the the result was the same when the expression of PCNA in the Hp positive group and Hp negative group on the expression of PCNA(p＞0.05).In SSQD group,the result was the same between the Hp positive group and Hp negative group(p＞0.05).Conclusion:1 The degree of atrophy,intestinal metaplasia in the SSDH group was much worse compared to it in the SSQD group and there was relationship between the difference and the existnace of Hp.2 Raised Erosive Gastritis patients whoses syndromes meet with spleen stomach-dampness-heat(SSDH) exist p53 over-expression and there was relationship between the difference and the existnace of Hp.3 Raised Erosive Gastritis is in a highly proliferative condition...