Siwutang Of Blood Vascular Endothelial Injury In Rats Intervention Role

Objective: To observe the Siwutang type of blood deficiency rats the effects of vascular endothelial role Siwutang blood deficiency in the clinical treatment of patients who proceed from the vascular endothelial and provide a theoretical basis and thus be better able to use in clinical Siwu soup. Methods: subcutaneous injection of acetyl phenylhydrazine (APH) caused by blood-type rats were observed Siwutang on rat tissues and blood nitric oxide (NO), endothelin (ET) and calcitonin gene-related peptide (CGRP) the impact of: (1) the specific processes and methods of the experiment: first a five-day pre-oral drug delivery. Dosage: high-dose treatment group was administered 6ml/kg, treatment group the volume of low-dose oral administration of high dose to the whole half side and diluted twice for such capacity, the normal control group and model control group give distilled water in such capacity. Start from the sixth day, while continuing to administration, while the model with the start of drug-treated subcutaneous injection of 1% acetyl phenylhydrazine (APH·NS), day time, a total of five doses of 100,50,50,30,30 mg/kg, subcutaneous injection of normal control group the same dose of normal saline. 14 days from the beginning to 0.4% sodium pentobarbital in batches of abdominal aorta after anesthesia animal blood, and the animals were killed from liver,lung, adrenal gland and other tissues after tissue homogenate, low-temperature centrifugation, and then taking the supernatant Liquid twenty degrees below zero on the refrigerator to preserve, after the end of animal experiments to be sent to the General Hospital of Jinan Military Region Detected organizations and serum nitric oxide (NO), endothelin (ET) and calcitonin gene-related peptide (CGRP). (2) statistical methods: the results listed in the data are x±s said that those engaged in analysis of variance and t test. Statistical software for spssl3.0 version of P <0.05 there is significant difference. Results: (1) the general condition of animals: three days after the start of modeling, the model group of animals began to appear lazy move, erecthair,deprivation,weight loss,claws pale lips, and so turn a blind eye or even blind situation, the treatment group with weight decreased, the overall status better than the model group. (2) nitric oxide (NO), endothelin (ET), calcitonin gene-related peptide (CGRP) effects: in the model group, ET/CGRP and NO/ET ratio imbalance Siwutang group significantly to improve this ratio imbalance. Conclusion: The experimental results show that Siwutang of vascular endothelial injury can be an effective regulatory role.