| Objective:To study the expression of vascular endothelial growth inhibitor (VEGI, TL1A), VEGF, TNF-α, and IL-1β in diabetic rats’ serum, vitreous and retina, and investigated its role in the pathogenesis of DR.Methods:70Wistar rats were randomly divided into controle group, diabetes mellitus (DM)1month group, DM3month group, and DM6month group. Cytokines in serum and vitreous were determined by ELISA, and cytokines in retina were examined by Immunohistochemistry-Paraffin section, Western Blot was used to detect the expression of VEGI, VEGF, and VEGFR-2in retina, hematoxylin-eosin staining was used to estimate the pathological change of DR. The results were analysed by one-way analysis of variances, independent samples t test and LSD test, the level of significance was set at P<0.05.Results:Levels of VEGI in serum were lower in DR groups compared with CON group (P<0.05), TNF-α and IL-1β increased in DM groups (P<0.05), and VEGF shows no difference (P<0.05). Vitreous VEGI in DM groups was lower than control group (P<0.01), but VEGF, TNF-α and IL-1β increased in the DM groups (P<0.05). Retina VEGI of DM1month and3month group were lower than control group (P<0.01), while no difference was showed between CON and DM6groups (P>0.05). Retina VEGF, TNF-α and IL-1β were significantly higher in DM group (P<0.05). Western Blot showed that the level of VEGI in DM1month group was significantly lower than the control group, while DM3and6month group were higher than the DM1month group, and levels of VEGF and VEGFR-2were higher in the DM groups. HE stain of retina pathological showed that no typical change in retina was presented until6-month DM foundation.Conclusion:VEGI plays a role in the pathogenesis of DR, which provides a new direction for the study of DR. |
