Association Of The ²⁹T>C Polymorphism Of Transforming Growth Factor-β1 Gene With Spinal Fusion

Objective:To explore the association of the first exon ²⁹T>C polymorphism of transforming growth factor-pi in peripheral blood gene with spinal fusion,so as to find out the genetic factors on bony fusion after bone transplantation.Methods:Totally 210 patients,of which 90 cases were received anterior cervical interbody fusion with autogenous iliac bone,120 cases were carried out posterior lumbar intertransverse bony fusion with vertebra,and 200 normal controls were included in this study.DNA was extracted from the venous blood and analyzed by allele-specific PCR and gene sequencing methods to detect The first exon ²⁹T>C polymorphism of TGF-β1 gene;X-ray examination were done after operation at 1 week,3,6,12 months in all the patients,and according to the lateral and dynamic view to evaluate the degree of bony fusion;Database was established to find out the correlation between the first exon ²⁹T>C polymorphism of TGF-β1 gene and bony fusion.Results:In all the 410 cases,127 patients(69 in case group,and 58 in control group) were found the T/T homozygote at the position 29 of Exonl of TGF-β1 gene,and 185 cases(98 in case group,and 87 in control group) were T/C heterozygote at the same positon of Exonl;and 98 cases(43 in case group,and 55 in control group) were C/C homozygote at the same positon of Exonl;There were the ²⁹T>C polymorphisms of TGF-pi gene all in two groups。There was no significant difference between the distribution of the ²⁹T>C polymorphisms of TGF-pi gene in two groups(P=0.707).In the cervical group,the fast group was 29 cases,medium was 36 cases,slow was 25 cases,which were correlated with the distribution frequency of T/T,T/C,C/C(P=0.001),and the distribution frequency of T/T in the fast group was higher than that in medium and slow group;In the lumbar group,the fast group was 39 cases,medium was 55 cases,slow was 26 cases,which had no relationship with the distribution frequency of T/T,T/C,C/C(P=0.647)。Conclusions:In our experiment,there were the ²⁹T>C polymorphisms of TGF-β1 gene all in two groups;and its distribution frequency is correlated with the postoperative bony fusion degree in the anterior cervical interbody fusion with autogenous iliac bone,and not correlated with that in posterior lumbar intertransverse bony fusion with vertebra.Several aspects should be considered,for example,more influencing factors will effect the intertransverse bony fusion degree than intervertebral fusion,and the evaluation standard is also more complicate in the intertransverse bony fusion,which should be investigated further.The results obtained have provided a theory foundation for the further study of genetic factors on bony fusion after bone transplantation.