| Objective By detecting the association of single nucleotide polymorphism(SNP)of rs4803455(TGF-β1),rs2284792 and rs3917201(TGF-β3),this study aims to investigate the relationship between the polymorphism of 3 SNPs(rs4803455,rs2284792 and rs2284792)and hereditary susceptibility of gestational diabetes mellitus(GDM)in Han population of Shandong,so as to provide the theoretical basis for detecting the pathogenesis of GDM.Methods We selected genes that closely related to islet function and blood glucose homeostasis as candidates and screened SNPs those with a minor allele frequency(MAF)value >5%in the Chinese Han population(CHB).This study recruited randomly 919 GDM patients that without serious complications as GDM group and 1177 healthy pregnancies in the same period as control group.All subjects were recruited from the Qingdao University Affiliated Hospital and Linyi people’s Hospital that hospitalized during October 2016 to July 2017.Pregnancise with other complications or history of blood transfusion or immunotherapy were excluded.In this study,2m L of the peripheral venous blood was collected routinely from all subjects,EDTA anticoagulant.It was fully mixed and then 300 ul whole blood was taken with 1.5 m L EP tubes.The genomic DNA was extracted within 48 h and placed in the-20℃ refrigerator.Genotypic distribution of all SNPs polymorphisms both in TGF-β1 and TGF-β3 were detected by Taqman Employing Quantitative Real-time PCR.The Hardy-Weinberg genetic balance was utilized to test the group representativeness of the controls.The differences in general clinical data of the two groups were analyzed by the student’s t-test or nonparametric test.The genotype and allele frequency distribution between the two groups were tested by the Pearson-X~2.The degree of relative risk was shown by the odds ratio(OR)and 95% confidence interval.There was significant statistical difference when the bilateral P-value less than 0.05.Result The subjects of the control group enrolled in the study,were in accordance with Hardy-Weinberg equilibrium(HWE)for all SNPs and displayed a group representative at the significance level of P-value >0.05.No significant difference was found in time of maternal age,gravidity and the number of abortion(P >0.05)between the cases cohort and the controls.However,weeks of admission and delivery of GDM group intended to be earlier and the weight gain of newborns was heavier(P <0.05).No statistical difference was detected either in rs4803455 or rs3917201 between these two groups in terms of genotypic frequencies(For rs4803455 χ2 = 1.206,P = 0.547;For rs3917201 χ 2 = 0.303,P = 0.859),nor for allelic frequencies(For rs4803455 χ 2 = 0.089,P = 0.766,OR = 1.019,95% CI 0.899-1.156;For rs3917201 χ 2 = 0.218,P = 0.641,OR = 1.029,95% CI 0.911-1.163).Meanwhile,statistical difference of genotype was found of rs2284792 in TGF-β3 between two groups(X~2 = 9.064,P = 0.011),but not the allelic frequencies(χ2 =1.592,P = 0.207,OR = 1.082,95% CI 0.957-1.224).When categorized into AG+GG and AA subgroups,differences between these twosub groups were detected(X~2 = 6.314,P = 0.012,OR = 1.270,95%CI 1.054-1.530).Conclusion The experimental results showed that polymorphisms of rs4803455 in TGF-β1 gene and rs3917201 in TGF-β3 gene were not associated with the development of the GDM in Shandong Han women.Conversely,rs2284792 in TGF-β3 gene might be involved in the progress of the GDM in Shandong Han women.The AA genotype of TGF-β3 might confer protection against GDM. |
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