Expression Of IL-12 And IL-4 In Nasal Polyps

Objective: To study immune status,activated level and characteristic of Th1/Th2 of T lymphocytes infiltrating nasal polyps by analyzing the expression of surface markers and cytokines secretion. By this research,possible helps for searching the pathogenesis and clinical treatment were hoped to be offered.Methods: 30 cases of nasal polyps and 20 cases of normal middle turbinate mucosa were involved in this study.The fresh samples were prepared into single cell suspension for flow cytometry. The concentration of IL-12 and IL-4 were determined by enzyme linked immunosorbent assay(ELISA).Results:Th1 subset was predominant in all the nasal polyps and the Th1 and Th2 subset in nasal polyps was significantly higher than that in normal inferior turbinate mucosa(controls);The concentration of IL-12 and IL-4 in nasal polyps tissues were significantly higher than that in controls(P<0.05); There was positive correlation between the mean percentage of Th1 subset and IL-12 in nasal polyps and positive correlation between the mean percentage of Th2 subset and IL-4 in nasal polyps; There was negative correlation between the percentage of IL-4 and IL-12 in nasal polyps.Conclusion: There was generous of T lymphocytes infiltrating in nasal polyps which indicated that there were disorder of immunological function and abnormal immunological activity in the local area. The mixed Thl/Th2 cytokines pattern was found in nasal polyps,which were possibly related to the establishment of the microenvironment and the mucosal immune system. Th cell of nasal polyps were differentiated into Th1 and Th2 by The effect of IL-12 and IL-4 seperatively , both of them may play a certain role in The occurrence and development of this disease.