The Role Of IL-17in Nasal Polyps And Allergic Rhinitis

Part â…  Effects of IL-17on expression of GRO-a and IL-8in fibroblasts from nasal polypsObjective: Recent studies indicated that interleukin (IL)-17, growth-related oncogene (GRO)-a and interleukin (IL)-8play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17and the production of GRO-a and IL-8in human nasal polyp fibroblasts are not completely understood. This study aims to determine the effects of the increased IL-17on the changes of GRO-a and IL-8expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps.Methods: Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of GRO-a and IL-8.Results: The mRNA of GRO-a and IL-8was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-a and IL-8produced by fibroblasts were increased gradually with the increases in IL-17concentrations.Conclusion:The present study showed that nasal fibroblasts can produce GRO-a and IL-8, and their production is remarkably enhanced by IL-17stimulation, thereby clarifying the mechanism of the IL-17mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps. Part â…¡ Expression and Significance of Interleukin-17and C CN1in Murine Allergic RhinitisObjective: IL-17is involved in a variety of inflammatory diseases, especially nasal polyps and allergic rhinitis. CCN1plays important roles in pathologic and physiologic processes, such as inflammation reaction, wound healing, angiogenesis and tumor. CCN1becomes a research hotspot in various diseases. Recently, domestic and overseas scholars gradually seem to attach importance to the relationship between IL-17and CCN1and allergic diseases in the upper respiratory tract, and its effect in the pathogenesis of allergic disease. This study aims to determine IL-17and CCN1expression in allergic rhinitise mice to explore the effective role and significance in allergic rhinitis.Method:6-8weeks of female BALB/c mice were divided into2groups: AR model group of intraperitoneal injection given0.4mg/ml OVA physiological saline with adjuvant Al(OH)3in1,8,15days,22-28days for7consecutive days nasal drops with25mg/ml OVA physiological saline. Control group given physiological saline. The expression of IL-17and CCN1was investigated by immunohistochemical analysis and RT-PCR, respectively.Results: The results of HE stain showed that the AR group had a large number of eosnophils cell infiltration and obvious epithelial thickening and glandular hyperplasia. The control group had no eosinophil cells infiltration and no epithelial thickening and glandular hyperplasia. The positive staining of IL-17and CCN1was mainly found in the nasal mucosa epithelial cells, glandular epithelium cells and inflammatory cells by immunohistochemical analysis. The mean IOD of IL-17and CCN1in the samples of allergic rhinitis was significantly higher than those in the control (P<0.05). There was a significant correlation between the protein levels of IL-17and CCN1(r=0.954, P<0.05). The levels of IL-17and CCN1mRNA in the AR group were significantly higher than that in the control group (P<0.05). There was a significant correlation between the protein levels of IL-17and CCN1(r=0.843, P<0.05).Conclusion:IL-17and CCN1expression increased in murine allergic rhinitis, and may participate in the inflammatory process of allergic rhinitis, and they have certain correlation in the pathogenesis of allergic rhinitis. These findings might provide a rationale for the treatment of allergic rhinitis.