Mesenchymal Stem Cells Transplantation On Arthrophlogosis And Serum TNF-α,IL-17 In Collagen-Induced Arthritis Rats

BackgroundRheumatoid arthritis(RA) is a mutilation autoimmune disease.Cytokines play important effects on enlarging immunologic derangement and joints destroy.TNF-a and IL-17 are representative cytokines,who make joints chronic symmetry corrodibility change and vasculitis.The topic was designed by mesenchymal stem cells transplantation in CIA rats,to investigate its possible immune recovery and immunomodulation mechanism.ObjectiveInvestigate the possible effect of mesenchymal stem cells transplantation on collagen-induced arthritis Rats.Investigate its possible synergistic effect and evaluate the possible mechanisms through the arthritis index,arthritis swelling degree and the serum TNF-a and IL-17.Methods1.50 Wistar rats were raised,received two times mixed liquor ofcattleâ…¡collagen and Freund's complete adjuvant(CFA) by endermic injection.CIA rats were made.2.40 CIA rats were divided into 5 groups randomly,and received mesenchymal stem cells transplantation treatment or other kinds control groups respectively.3.Observe the changes of arthritis index,arthritis swelling degree,and appearance of imageology and pathology.Take blood,separate and obtain serum, then detected the serum TNF-a,IL-17 by enzyme-linked immunosorbentassay.Results1.Arthritis index,arthritis swelling degree,and appearance of imageology and pathology are labels,42 rats who had received mixed liquor of cattleâ…¡collagen and CFA happened symmetry polyarthritis;2.The early treatment group and advanced stage late treatment group can inhibit the appearance of arthritis and serum TNF-a,IL-17 of CIA rats,the difference has statistics meaning.The early treatment group can inhibit arthritis index,arthritis swelling degree,and serum TNF-a,IL-17 of CIA rats obviously,the difference between mesenchymal stem cells transplantation group and model group has statistics meaning(P<0.05).3.By mesenchymal stem cells transplantation in CIA rats,it has remarkably effect in inhibiting arthritis index,arthritis swelling degree,and serum TNF-a,IL-17 of CIA rats(P<0.05 ).Conclusions1.The mesenchymal stem cells transplantation in CIA rats has remarkably effect in inhibiting arthritis index,arthritis swelling degree,and serum TNF-a,IL-17 of CIA rats.2.It indicated that the mesenchymal stem cells transplantation in CIA rats has the functiong in immunomodulation.