The Effect Of Human Umbilica L Cord Mesenchymal Stem Cells Transplantation On Serum Cartilage Oligomeric Matrix Protein In Rats With Collagen-Induced

BackgrounBackgroundRheumatoid arthritis (RA) is a highly disabling autoimmune disease. Inflammatory cytokinesplay an important role in enlarging systemic immunologic dysfunction and local joint destroy.COMP is representative cytokines, eventually leading to chronic, symmetrical, erosive arthritisof peripheral joints and performance of extra-articular vasculitis. This topic was designed bymesenchymal human umbilicalcord derived-mesenchyma stem cells transplantation from the tailvein, to investigate its possible mechanisms in immune recovery and immunomodulation in CIAratsObjectiveTo observe the treatment of hUC-MSCs transplantation on animal models of CIA, to observejoint symptoms before and after transplantation, and expression of COMP levels in synovial andserum. To investigate the possible regulation mechanism of hUC-MSCs transplantationon.COMP.Methods1. SD rats were raised,and respectily given bovine collagen typeâ…¡and complete Freundadjuvant mixture by endermic injection for two times every tow week., CIA rats were made.2. The 40 rats were randomly divided into 4 groups: control group, CIA control group, MSCstreatment group, MTX treatment group;3. Observed symptoms (including the general concept, arthritis index, swelling joint) and thechanges of pathology images on four time points in CIA rats,.Took blood of rats and detected thelevel of serum COMP at different time points.Results1. Through the symptoms, pathology images,33 rats occured symmetric polyarthritis in 40 ratsreceived bovine collagen typeâ…¡and mixture of Freund's complete adjuvant sensitized.Theoverall rate of modeling success was 83% .2. By variance analysis of arthritis index, repeated measures of swelling joint data and observedchanges of image pathological ,we confirmed the model group had significantly higher damagethan the control; Joint symptoms improved more significantly in the treatment group ofmethotrexate and the treatment group of MSCs transplantation.3. Through analyzing the serum levels of COMP in every groups,we found that MSCs transplantation inhibited the level of COMP in CIA rat.conclusioconclusion1. MSCs transplantation can reduce arthritis symptoms.2. MSCs transplantation significantly decreased the expression levels of synovial and serumCOMP in CIA rat.