Investigation On Pathomorphology Of Meynert Basal Nuclei Nerve Cell In Depression Models Of Different Cotemporary Sd Rats

Objective:the topic intent to initial approach central cholinergic system place of origiin Meyner basal ganglia of pathomorphism changing and the transmitter Ach transmutation in the courage of depressive disorder,and to approach discrepancy of difference age group.Methods:Selecting cleaning maleness SD rat 120,4-5week 40,4-5 month40,14-15 month 40,through Open- fieldtest each choosing the nearer 32.random average enter control group and model group,each is 16. Utilize chronic light unpredictable stimulus to make depressive rat model,through Open- fieldtest and sacchar favor experiment detect praxiology.Using TEM observe the changing of Meynert basal ganglia neuropil syaptic.Using CHAT immunity class observe the changing of cholinergic neuron:Applying flow cytometry and TEM observe to neure Apoptosis.Results:Comparing the stress rat between in the Open- fieldtest and sacchar favor experiment,the difference contrast of experiment is notable,and the 14-15 group each items all nobale infra others.TEM oberservation show that the quantity of model group synaptic vesicle is obviously less than controle group,and is positive correlation to age and synaptic vesicle maldistribution,assembling activated synaptic ribbon,all age model group CHAT immune reaction more strength than CG.Flow cytometrer combine with TEM show:model group nerve cell Apoptosis obviously more increasing than controle group,with the growing nerve cell Apoptosis obviously increasing.Comparing 14-15 month model group with 4-5 week model group and 4-5month model group has statistical significance.Conclusions:1 Depressive disorder model rat central cholinergic system place of origiin Meyner basal ganglia neuropil synaptic vesicle obviously reduction,while CHAT immune reaction positive cells obviously increasing,and close to the age.2 Depressive disorder model rat Meyner basal ganglia nerve cell exist obviously Apoptosis,and with growing cell Apoptosis obviously accelerate.3 Central cholinergic system place of origiin Meyner basal ganglia and depressive disorder are in relevantion,which study is important part of depressive disorder nosogenesis.