| ObjectiveThe purpose of this study was to detect the expression of adiponectin in serum, and adipose tissue of patients with preeclampsia(PE), analyze the relationship between adiponectin with serum lipid and insulin resistance, discuss their roles in the pathogenesis of PE.MethodsThe levels of adiponectin and insulin in serum were detected by enzyme-linked immunosorbent assay, the levels of lipid and glucose were detected with biochemistry analyzer in nonpregnant healthy women(n=30), in normal third-trimester pregnant women(including pregnant week≤34W group(n=30) and pregnant week>34W group(n=30)), and in cases of women with early-onset PE(n=29) and with late-onset PE(n=31).The adiponectin mRNA expression levels in subcutaneous and omental adipose tissues of women with early-onset PE(n=24), late-onset PE(n=25) and control pregnant women(n=30) were determined by real-time fluorescent quantitative polymerase chain reaction.Results①In early-onset and late-onset PE groups, the levels of serum adiponectin were lower than that of their controls, the level in early-onset PE group was lower than that in late-onset PE group. The differences were still significant after correction for haematocrit(P<0.05 in all).②In early-onset and late-onset PE groups, the levels of total cholesterol(TC), triglyeride(TG),low density lipoprotein cholesterol(LDL),apolipoprotein B(ApoB), insulin and insulin resistance index(IRI) were higher than that of their controls, while high density lipoprotein cholesterol(HDL) and apolipoprotein A1(ApoA1) were lower than that of their controls (P<0.05 in all).③In early-onset and late-onset PE groups, the serum adiponectin was negatively related to the serum TG, systolic pressure(SP), diastolic pressure(DP), mean arterial blood pressure(MAP) and IRI, while the serum apdionectin was positively related to the serum HDL.④Adiponectin mRNA expression was significantly lower in omental tissue in early-onset and late-onset PE groups than control group, and the level in early-onset PE group was lower than that in late-onset PE group(P<0.05); While compared their expression in subcutaneous adipose tissue with control group, there was no significance(P>0.05).⑤In early-onset and late-onset PE groups,serum adiponectin was positively related to omental and subcutaneous adiponectin expression, the correlation with omental adiponectin expression was higher than with subcutaneous adiponectin expression.⑥In early-onset and late-onset PE groups, the omental adiponectin mRNA level was negatively related to serum TG and IRI.Conclusions①Decreased adiponectin expression in serum and omental adipose tissue may be involved in the pathogenesis of preeclampsia, especially in the pathogenesis of early-onset PE.②Decreased expression of adiponectin in omental adipose tissue contributes to low serum adiponectin level of PE.③Dyslipidemia and insulin resistance may be associated with decreased adiponectin expression in patients with PE, and may be involved in the pathophysiology of hypoadiponectinemia led to development of PE. |
