Anti-inflammatory Liquid Combined With Various Means Extract From Rabbit Skin Inflamed By Vaccinia Virus For Injection On Rat Sciatic Nerve Injury Repair

Objective: In particular, peripheral nerve injury are caused by chronic compression of the sciatic nerve injury are common clinical features restore the extent of a direct impact on patient quality of life, so how to promote damage to nerve regeneration and repair of clinical research has been important topics。The impact of nerve regeneration and repair a number of factors, one of the Agency's micro-environment injury is the most important influencing factors。Has been commonly used anti-inflammatory analgesic on micro-environment plays an important regulatory role。With the Extract from Rabbit Skin lnflamed by Vaccinia V irus for lnjection, ERSVV more and more treatment for nerve repair, Its route of administration from intramuscular injection, intravenous infusion of the celiac plexus, has been widened subarachnoid。SD rats used in this experiment, in the light of Bennett and Xie Designed chronic compression of the sciatic nerve model to explore the anti-inflammatory combined with liquid ERSVV,ERSVV in damaged peripheral nerve direct delivery of the role of nerve repair is superior to intramuscular injection。Designed to provide a basis for clinical treatment.Methods: 80 male SD rats, weighing 280 +20 g, were randomly divided into 4 groups of 20。.A was the sham group,B was the CCI group , C group was given anti-inflammatory analgesic perineural fluid, muscle injection ERSVV, D group perineural given fluids and anti-inflammatory analgesic ERSVV. The rats were anaesthetized with 10% chloral hydrate(300mg/kg),made the middle femoral incision. The sciatic nerve of the rats in the A group were exposured only, no ligation. Rats with right sciatic nerve been ligated and placed the epidural catheter were used as group B,as group C,group D.This three group were injected with water for injection by the epidrual catheter.After CCI fourteen days, rats pain-related behavioral response was irritability.Sciatic nerve was extracted and observed with light microscope. The sciatic nerve were edema and axon number reduced and medullary sheath were disaggregated. CCI model rats were successfully constructed. Group B continued to 14 days after the transcatheter injection 0.2ml water for injection until subjects。C, D groups at 14 days through the purchase of anti-inflammatory analgesic liquid injection pipe (5mg/ml compound betamethasone 0.05ml +2% lidocaine 0.05ml +0.5 mg / ml vitamin B120.05ml + sterile water 0.05 ml)。From the first 15 days only, C group every day injection mixture 0.2 ml (water for injection 0.1ml +2% lidocaine 0.05ml +0.5 mg / ml vitamin B120.05ml), while intramuscular injection ERSVV0.1ml. D Group is to add at ERSVV 0.1ml water for injection mixture in the alternative, until the material. CCI separately in four groups after the first 14 days, 21 days, 28 days measurement of sciatic nerve function index (Sciatic functional index, SFI); and at random from each group 7 rats, the sciatic nerve exposed under anesthesia to observe the neural changes in the general ; measuring nerve conduction velocity (nerve conduction velocity, NCV) and amplitude (Amplitude, APT) and materials,At light and electron microscopy of the ultrastructure of sciatic nerve, design analysis and calculation for the number of axons and myelin thickness; by immunohistochemical method to observe NGF (Nerve growth factor, NGF) Change the number.Results:1.SFI A group of about -0.2 at different time points no significant change. Group B maintained at about -33. C, D group were higher than the 21 days group B and lower than the A group; the first 28 days of C group was significantly greater than the B group (P <0.05); D group than the B group was significantly (P <0.01); D Group significantly greater than the C group (P <0.05); comparison group C, D groups of 28 days was superior to 21 days (P <0.05), more obvious than 14 days (P <0.05).2.NCV;AMP With the A group, B, C, D the first 21 days of the three groups was significantly lower (P <0.01); the first 28 days decreased significantly (P <0.05). 21,28 days, C, D groups were higher than those in B group (P <0.05); D group was significantly higher than C group (P <0.05); C, D groups, respectively, and 28 days, 14 days for comparison group or high very significant (P <0.05; P <0.01). 3.Axon count and myelin thickness C, D Group 21,28 days than B group than in A group; D group was significantly greater than the C group (P <0.05); C, D groups, respectively, and 28 days, 14 days for comparison group was significantly higher (P <0.05; P <0.01).4.Immunohistochemistry A group of non-expression or a small amount of NGF expression. B, C, D, E Group nerve fiber degeneration and regeneration of the axon and Schwann cells (SC) have a certain number of membrane expression。Image analysis results showed that :Department nerve injury in the average gray-scale index: group than, B, C, D Three groups of 28 days more than 21 days and 14 days; but no significant Group B; 21,28 days C, D groups were large in the B group (P <0.05; P <0.01), D group than the C group (P <0.05).5.General and histopathological observation Gross observation A group of nerve-smooth, with the surrounding tissue without adhesion,Model after 14 days, B, C, D in each group significantly sciatic nerve hyperemia, edema, thickening, adventitial color than red. 21,28 days, group B was not obvious to mitigate, C, D Group hyperemia, edema significantly reduced, the outer membrane of the white color than the 14 days。D Group hyperemia, edema significantly lighter than the C group。Observed under light microscope A group of nerve fibers were arranged regularly, intact myelin。The first 14 days B, C, D group with nerve fiber disorders, loss of myelin nerve edema serious reduction in the density of nerve axons, showing off many of myelin。B group at 28 days of nerve fibers and axons are arranged in an increase in the number, but with C, D groups compared the same time there is significant difference。C, D groups of nerve fibers with a more orderly, better direction, Schwann cell proliferation significantly, nerve fibers grow better, and D group was significantly better than the C group, microscope myelin thickening, increase in the number of axons。The above-mentioned cases of nerve repair 28 days C, D were significantly better in 21 days. Electron microscopy At the first 14 days, A group of neural integrity, structure rules; section 28 days B Group axons part fracture, spinal fusion disappeared, microfilaments microtubules to reduce。C group disappeared most spinal fusion, tubulin microfilaments can be seen, a lesser injury, myelin thickening, lamellar change the rules, but the D group has the number of myelinated nerve fiber, axon diameter, myelin thickness, axon within mitochondria, microfilaments, microtubules, etc. Quantity is greater than D group. C, D to repair the situation of the two groups were significantly better in 21 days.Conclusion: 1 Combined with liquid anti-inflammatory analgesic conducive ERSVV nerve injury repair2 ERSVV perineural administration of the repair role of damaged neurons better than the intramuscular injection.