Craniocerebral Injury Effects On The Repair Of Peripheral Nerve Injury

Craniocerebral injury can promote fracture healing. Research shows that the body of cytokines, neuropeptides and neurotrophic factors, humoral factors and mechanical factors change after craniocerebral injury, and promote the healing of fractures. At the same time has been reported, neurotrophic factors promote nerve repair and regeneration. So whether changes in craniocerebral injury after neurotrophic factor affects peripheral nerve repair and regeneration, no relevant reports.This study seeks to contact craniocerebral injury and peripheral nerve injury and repair between related causes further explore the mechanism and pathophysiology of craniocerebral injury, as well as the impact, while the expansion of the theoretical basis of peripheral nerve repair and regeneration, clinical and treatment.Objective:To study the effects of craniocerebral injury on the repair of peripheral nerve injury, and the mechanisms that affect.Methods :1 Grouping and preparation of animal models80 male SD rats were randomly divided into A, B groups, A group for the experimental group, using the classical method of manufacturing free-fall device Feeney, hit the right brain produce moderate craniocerebral injury in rats, while on the left side of rats 1cm below the piriformis sciatic next hole cut, apply 9-0 noninvasive epicardial suture suture line. Group B was the control group, only after the left sciatic nerve cut suture.2 The index measuring the sciatic nerveHomemade rat walking obscura, measurements were done footprints 4,6,8,12 weeks after surgery, the measured values into the formula, and seek sciatic functional index(SFI). To 0:00 SFI value for normal nerve-100 completely broken off.3 gastrocnemius wet weight measurementsRespectively, after modeling 4,6,8,12 weeks, each group were 10 rats after intraperitoneal injection of chloral hydrate anesthesia death, since the femoral condylar calcaneal tubercle starting to remove dead complete gastrocnemius, electronic weighing scales accurate wet muscle mass. Gastrocnemius wet weight recovery rate(%) = experimental side wet weight(g) / normal side of the wet weight(g) × 100%.4 HE stainingSelect the sciatic nerve stump, after HE staining in modeling, 4,6,8,12 weeks after. Changes observed nerve fibers under an optical microscope.5 Masson stainingAfter eight weeks in modeling and 12 weeks, patients taking oral nerve ends of 0.5cm, slice thickness of about 3-5um, reference manual staining method to observe the nerve membrane collagen fibers were mounted case, the optical microscope.6 Immunofluorescence staining8 weeks and 12 weeks, whichever segment nerve nerve anastomosis mouth frozen sections, NF200 immunofluorescence staining in modeling. Under the fluorescence microscope neurofilament situation arise.7 horseradish peroxidase(HRP) tracer techniqueAfter modeling, 4,6,8,12 weeks, each group were 10 rats after intraperitoneal injection of chloral hydrate anesthesia, the left sciatic nerve stump at 0.5mm beyond, after a slight setback clip nerve, injection 30% HRP solution under deep anesthesia 72 h after thoracotomy, the left ventricle through the ascending aorta cannulation, rinse with warm saline 200 mL of blood vessels, and then with 2% paraformaldehyde and 0.1mol / L PBS 2% glutaraldehyde 400 mL fixative perfusion fixation, take sciatic spinal ganglia and the corresponding T4-5 spinal segment, cross-sectional slices of continuous oscillation 50μm. OK benzidine(BDHC) staining, neutral red dye. Light microscope and the number of ganglion cell bodies of spinal cord anterior horn motor neurons containing blue dye particles.8 TEMAfter eight weeks in modeling and 12 weeks after, drawn to the nerve anastomosis centered trimmed to 2mm × lmm × 1 mm tissue blocks, fixed, embedded, stained newborn myelin ultrastructure and organelle morphology by transmission electron microscopy.The result:1 Effect of craniocerebral injury in rat sciatic functional index on the surrounding nerve injuryBlack-box test results show that the first four weeks, two groups of rats after modeling near SFI(P> 0.05); SFI rats began to decrease from the first four weeks injury group, with time to reduce the magnitude of slowing, while the control group of rats SFI started to decline from the first six weeks; 8 and 12 weeks, the rats were SFI injury group than the control group(P <0.01).2 Brain Injury surrounding nerve injury in rats gastrocnemius wet weight of influenceTwo experimental groups compared with the contralateral side of the rat gastrocnemius pale color, muscle atrophy, and 4 weeks after modeling when two groups of rats gastrocnemius wet weight recovery rate close to(P> 0.05), while a large group of 6-12 weeks of injury rat gastrocnemius muscle recovery rate was significantly higher(P <0.01).3 Craniocerebral injury in rats around the sciatic nerve injury affect the pathological changesHE staining showed that at 4 weeks after modeling, injury group, the control group had no significant difference between the two groups, and no nerve fibers through the stump, stump nerve disorders, no intact nerve fibers, but appear more visible proximal nerve injury group fibroblasts, whereas the control group, a large number of vacuoles around necrotic cells. 6 weeks, the damage through nerve fibers were observed in the stump, but less sparse, uneven thickness, disorganized; the control group had no nerve fibers through the surrounding cells are still more common vacuolar degeneration, some nerve adhesions, broken beyond the finer end. At 8 weeks, the group can be seen more damage nerve fibers through the stump, thickness become more consistent, more neatly arranged, but the nerve fibers are thin; the control group also visible through the ends of nerve fibers, but uneven thickness, disorganized, some nerve adhesions, smaller ends beyond. 12 weeks, the injury group showed a larger number of nerve fibers through the stump diameter thick, arranged in the same, no significant difference between the normal fiber; control group, there are more nerve fibers through the stump, the same thickness, has shown a typical nerve fibers wavy arrangement.4 Masson trichrome8 and 12 weeks after surgery, endoscopic were seen in the control group was significantly more than the collagen fibers in the injured group anastomosis. And disordered rare nerve fibers, nerve fibers lose Traveling wave-like structure.5 Immunofluorescence staining8 and 12 weeks after surgery, the experimental group in the density of nerve regeneration, as well as aspects of the arrangement degree myelin thickness rules are superior to the control group, the experimental group showed nerve damage has been rapid repair and regeneration of nerve fiber regeneration high quality.6 horseradish peroxidase(HRP) tracer techniqueLight microscope, the first four weeks after modeling, the two groups of rat sciatic nerve ganglia, spinal cord segments corresponding angle before were not found in blue-black marked neurons, showing a lot of swelling and cell; six weeks, the damage rat sciatic nerve cell bodies in the control group ganglion in no visible labeled neurons; 8 weeks, sciatic nerve injury in rats ganglia, within the corresponding anterior horn spinal segment can be seen clearly labeled cells, an average of 12 less-15 / field, the control group, the average 2-5 / field; 12 weeks, two groups were seen clearly labeled cells.7 TEMAfter 8 weeks, 12 weeks both groups have different numbers of myelinated nerve fibers, and after 12 weeks compared with the number of myelinated nerve fibers more than eight weeks, the structure clearer, more distinct layers, more particularly close to normal nerve injury group structure. Experiments can also be seen after 8 weeks, 12 weeks injury group regenerated myelinated fiber number, hierarchy of clarity, as well as the arrangement of organelles myelin recovery were better than the control group.Conclusion:Craniocerebral injury in a certain degree of peripheral nerve repair with the promotion of the role of head injury can reduce the sciatic nerve scar healing, promote nerve fiber growth, accelerate the maturity of the myelin sheath, near and promote faster recovery of neural morphology and function.