| BackgroundIschemic stroke as one of clinical risk,urgency disease,takes trouble not only to patients but also to their families.In our country,with the development of social and economic,the incidence of this disease increased year by year,so in-depth study of the pathogenesis of stroke,and effective treatment is a common goal of scientific research workers and clinical workers. Acupuncture treatment of stroke in China has thousands of years history,as early as the"Canon","A and B Acupuncture classics"there are relevant records, particularly in the recovery of limbs functions,such as physical function, swallowing function and so on,which are still widely used by clinical.Experimental studies have confirmed that acupuncture can improve cerebral ischemic injury.This experimental based on the ischemic injury,to observe local changes in the expression of JAK1,combined with the recovery of limb function,to verify the therapeutic effect of acupuncture and to explore the possible mechanism of the action.MethodsUsing the method of MCAO to make the model.Observing the changes level of Jak1 in the brain of cerebral ischemia rat and changes of behavior in 2 hours,24 hours,three days after injury,as well as electro-acupuncture Baihui(DU20),Dazhui(DU14),obseving the impact of these indicators and to explore the needle mechanisms.1.Take Berderson and Belayev's method,after 2 hours,24 hours and 72 hours accoding to limb movement,assessed in accordance with the 0-4 scores,the higher the score,the more serious injury.2.Through TUNEL method to check the apoptosis of nerve cells in each group.3.Using immunohistochemical detection method to check the level of expression of JAK1 after acupuncturing cerebral ischemia rats in 2 hours,24 hours and 72 hours, Observing the changes and effects.The results(l)Neurofunctional scores:there represent neurological deficits in different extent of model group and EA group.For lday,most have 2 marks of neurofunctional scores.The scores of model group discreased from 1day to 3days. There have significant differences of model group compared with sham-operated group from lday to 3 days(P＜0.05,P＜0.01).With the ongoing of brain injury, the neurofunctional scores of EA group decreased,and neurofunction improved.(2)Neural apoptosis index:There is no obvious apoptotic cells in Sham-operated group,in 2 hours there is no obvious apoptotic cells in the model group,in 24 hous we can see the obvious features of a large number of apoptotic cells, after 3 days the cells decreased;in the electro-acupuncture group there is no obvious apoptotic cells in 2 hours,but after 24 hours and 3 days we can see apoptotic cells but less than model group(P＜0.01).(3)the level of JAK1:There was no obvious expression of JAK1 in Sham-operated group;in 2 hours,there is no obvious expression in the model group,in 24 hours we can see expression increased,continuing to the third day;in 2 hours, there is no obvious expression in electro-acupuncture group,24 hours,the expression can be seen clearly,the third day,the model group was higher in expression(P＜0.05)ConclusionCerebral ischemia injury in rats can cause obvious signs of neurological deficit,and electro-acupuncture"Dazhui"(DU14)and"Baihui"(DU20)can improve neurological function in rats.in normal brain tissue there is no expression of JAKI protein,while the experimental rat model of cerebral ischemia have much obvious JAKI protein expression,after electro-acupuncture treatment in the experiment rat model the expression of JAKI increased, consider the electro-acupuncture treatment in experimental rat model can regulate the disorders of the rats by increasing the expression of JAK1.Its may be one of the mechanism that electro-acupuncture treatment the ischemic stroke.
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